ROLE OF PLATELET LIPIDS IN HEMOSTASIS AND THROMBOSIS

这项建议考察了花生四烯酸含乙醚的作用。 磷脂与血小板功能。该提案的主要重点是 研究(1)血小板转酰基酶的调节和性质 用于将花生四烯酸掺入乙醚磷脂和(2) 血小板磷脂酶A2，释放酯化花生四烯酸 用于后续转化为生物活性的磷脂 代谢物。该提案的主要目标是探索控制和 花生四烯酸在乙醚中代谢的功能意义 人类血小板中的磷脂。主要技术将包括方法 膜结合酶(洗涤剂)的提纯和分析 增溶、柱层析、电泳法)和方法 脂类混合物(薄层和高性能)的拆分和分析 高效液相色谱)。 这些研究将(1)提供对生化和功能的洞察 与生物活性生产有关的酶的性质 化合物(二十烷基类化合物和血小板激活因子)，(2)阐明 乙醚磷脂的生物学作用(S)和(3)对乙醚磷脂的进一步认识 出血性心绞痛患者的血小板功能及参与程度 血栓性疾病。

This proposal examines the role of arachidonic acid containing ether phospholipids in platelet function. The major focus of the proposal is to study regulation and properties of (1) platelet transacylase, responsible for incorporation of arachidonic acid into ether phospholipids and (2) platelet phospholipase A2, liberating esterified arachidonate from phospholipids for subsequent transformation to biologically active metabolites. The major goal of the proposal is to explore the control and functional significance of the metabolism of arachidonic acid in ether phospholipids in human platelets. Major techniques will include methods for purification and analysis of membrane-associated enzymes (detergent solubilization, column chromatography, electrophoresis) and methods for resolution and analysis of lipid mixtures (thin layer and high performance liquid chromatography). These studies will (1) provide insight into biochemical and functional properties of enzymes involved in production of biologically active compounds (eicosanoids and platelet activating factor), (2) elucidate the biological role(s) of ether phospholipids and (3) further the knowledge of platelet function and involvement of platelets in hemorrhagic and thrombotic diseases.