PLURICRINE REGULATION OF FOLLICULAR TGF-B EXPRESSION

卵巢卵泡发生的现代观点认识到 内分泌、旁分泌和自分泌因素对此的调节作用 进程。最近的兴趣集中在卵巢内生长的作用上。 卵泡细胞增殖与调控因子的研究进展 差异化。尤其令人感兴趣的是转化生长因子-β (转化生长因子-β)对细胞的多功能作用 增殖分化。推测卵泡膜上的转化生长因子-β 细胞来源调控猪卵泡发育 颗粒细胞和膜细胞的增殖和分化。膜细胞 转化生长因子-β的表达受内分泌因素和卵巢内的调节 增长因素。本项目将通过以下方式重点检验这一假说 猪卵泡膜细胞转化生长因子-β的分泌调控分析 不同的水平：(A)分泌和(B)使用高度敏感的 生物测定；(C)使用生物测定和放射性标记的细胞积累 氨基酸掺入和免疫沉淀；和(D)转录，通过 用特异的cDNA杂交技术分析转化生长因子-β基因的表达。使用 作为端点，我们将解决以下问题： (1)卵泡膜细胞是否产生转化生长因子-β，如果有，转化生长因子-β亚型是什么？ (2)促黄体生成素和/或促卵泡生成素 通过膜细胞直接调节转化生长因子-β的产生，在什么水平上？ (3)转化生长因子-α/表皮生长因子、血小板衍生生长因子、胰岛素样生长因子和转化生长因子-β的作用。 卵膜和颗粒细胞的增殖和分化，直接 调控卵泡膜细胞转化生长因子-β的产生，在什么水平？ (4)颗粒细胞是否调节卵泡膜细胞转化生长因子-β的产生？ (5)促黄体生成素、卵泡刺激素、转化生长因子α/表皮生长因子、血小板衍生生长因子、胰岛素样生长因子和转化生长因子-β是否通过颗粒细胞起作用？ 来调节卵泡膜细胞转化生长因子-β的产生？ 以上概述和此处详细说明的研究将提供一个全面的 一种强势生长的表达调控和激活分析 参与调节卵泡发育的因子。此外， 它们将提供一种方法来测试有关 生长因子在毛囊细胞功能中的自分泌和旁分泌作用。

The modern view of ovarian folliculogenesis recognizes the potential regulatory impact of endocrine, paracrine, and autocrine factors on this process. Recent interest has focused upon the roles of intraovarian growth factors in the regulation of follicle cell proliferation and differentiation. Of particular interest is transforming growth factor-beta (TGF-B) by nature of its multifunctional role with respect to cell proliferation and differentiation. It is hypothesized that TGF-B of theca cell origin regulates follicle development in the pig by regulation of granulosa and theca cell proliferation and differentiation. Theca cell TGF-B expression is in turn regulated by endocrine factors and intraovarian growth factors. This project will focus upon testing this hypothesis by analyzing the regulation of TGF-B production in porcine theca cells at four distinct levels: (A) secretion and (B) activation using a highly sensitive bioassay; (C) cellular accumulation using bioassay and by radiolabeled amino acid incorporation and immunoprecipitation; and (D) transcription, by analysis of TGF-B mRNA production using specific cDNA hybridization. With these as endpoints the following questions will be addressed: (1) Do theca cells produce TGF-B and if so what TGF-B subtype? (2) Do luteinizing hormone (LH) and/or follicle-stimulation hormone (FSH) directly regulate TGF-B production by the theca cells and at what level? (3) Do TGF-a/EGF, PDGF, IGF-I, and TGF-B, growth factors which regulate theca and granulosa cell proliferation and differentiation, directly regulate the production of theca cell TGF-B and at what level? (4) Do granulosa cells regulate the production of theca cell TGF-B? (5) Do LH, FSH, TGFa/EGF, PDGF, IGF-I and TGF-B act through granulosa cells to regulate theca cell TGF-B production? The studies outlined above and detailed herein will provide a comprehensive analysis of the regulation of expression and activation of a potent growth factor implicated in the regulation of follicle development. Moreover, they will provide a means with which to test statements with respect to the autocrine and paracrine roles of growth factors in follicle cell function.