CHARACTERIZATION OF HEART ATRIAL MUSCARINIC RECEPTOR

心脏的副交感神经支配，在心房中由 毒蕈碱型乙酰胆碱受体;已知参与 以及促进许多心律失常。 为了获得 深入了解毒蕈碱的分子机制和组成 受体，该蛋白质的纯化提出使用技术 例如亲和层析。 建议进行流体动力学研究， 为了获得有关表观分子量和形状的信息， 溶解的蛋白质。 热力学研究的影响， 提出了诸如鸟嘌呤核苷酸的调节剂， 确定这些化合物影响受体的机制 与毒蕈碱激动剂和拮抗剂的相互作用。 信息 这些研究的结果将提高我们的水平， 了解心脏的神经控制，并可能导致 开发更有效的抗肿瘤药物。

Parasympathetic innervation of the heart, mediated in the atria by the muscarinic acetylcholine receptor; is known to be involved in the genesis and facilitation of a number of cardiac arrhythmias. In order to gain insight in to the molecular mechanism and composition of the muscarinic receptor, the purification of this protein is proposed using techniques such as affinity chromatography. Hydrodynamic studies are proposed in order to gain information on the apparent molecular weight and shape of the solubilized protein. Thermodynamic studies of the effects of putative regulatory agents such as guanine nucleotides are proposed in order to determine the mechanism by which these compounds affect receptor interactions with muscarinic agonists and antagonists. Information obtained as a result of these studies will increase our level of understanding of neural control of the heart and may lead to the development of more effective antiarrhythmic agents.