CHARACTERIZATION OF HEART ATRIAL MUSCARINIC RECEPTOR

心脏的副交感神经支配，由 M胆碱型乙酰胆碱受体；已知参与了 以及促进一些心律失常的发生。为了获得 对毒扁豆碱分子机制和组成的洞察 受体，建议用多种技术纯化该蛋白。 例如亲和层析。水动力研究建议于#年。 以获得关于表观分子量和形状的信息 可溶解的蛋白质。推定影响的热力学研究 诸如鸟嘌呤核苷酸之类的调节剂的提出是为了 确定这些化合物影响受体的机制 与M受体激动剂和拮抗剂的相互作用。信息 这些研究的结果将提高我们的水平 了解神经对心脏的控制，可能会导致 开发更有效的抗心律失常药物。

Parasympathetic innervation of the heart, mediated in the atria by the muscarinic acetylcholine receptor; is known to be involved in the genesis and facilitation of a number of cardiac arrhythmias. In order to gain insight in to the molecular mechanism and composition of the muscarinic receptor, the purification of this protein is proposed using techniques such as affinity chromatography. Hydrodynamic studies are proposed in order to gain information on the apparent molecular weight and shape of the solubilized protein. Thermodynamic studies of the effects of putative regulatory agents such as guanine nucleotides are proposed in order to determine the mechanism by which these compounds affect receptor interactions with muscarinic agonists and antagonists. Information obtained as a result of these studies will increase our level of understanding of neural control of the heart and may lead to the development of more effective antiarrhythmic agents.