LUNG CELL FUNCTION IN HEALTH AND DISEASE

将研究低氧对糖酵解的协调遗传调控。 在细胞系统中，通过测量糖酵解酶含量， 生物合成和降解.特定信使核糖核酸水平的测量 糖酵解酶的种类及糖酵解率的测定 特定mRNAs的生物合成和降解。各式各样的细胞 将使用模型。 低氧对线粒体基因表达的调节将是 线粒体酶在分离细胞系统和器官中的研究 线粒体DNA和线粒体16S核糖体RNA的活性和含量测定。 成纤维细胞胶原代谢在基因水平的改变 高氧、低氧、博莱霉素和吞噬细胞将在不同的 成纤维细胞系统。 胶原蛋白生物合成协同遗传调控的可能性 将通过研究6种基因特定mRNA的变化来进行研究 翻译后酶。 此外，该提案还将用作医疗培训的形式。 学生、博士后博士后、博士后医学博士研究生和 通过单独资助博士后研究员。

The coordinate genetic regulation of glycolysis by hypoxia will be studied in cell systems by measurements of glycolytic enzyme content, rates of biosynthesis and degradation; measurements of levels of specific mRNA for the various glycolytic enzymes and measurements of the rates of biosynthesis and degradation of the specific mRNAs. A variety of cell models will be used. The regulation of genetic expression in mitochondria by hypoxia will be studied in isolated cell systems and organs by measurements of mt enzyme activity and content, measurements of mt DNA and mt 16S ribosomal RNA. Alterations in collagen metabolism in fibroblasts at a genetic level by hyperoxia, hypoxia, Bleomycin and phagocytes will be studied in various fibroblast systems. The possibility of coordinate genetic regulation of collagen biosynthesis will be studied by investigating alterations in specific mRNA for the 6 post-translational enzymes. In addition, the proposal will be used as a training format for medical students, pre-doctoral PhD candidates, post-doctoral MD fellows and post-doctoral PhD fellows through separate funding.