LUNG CELL FUNCTION IN HEALTH AND DISEASE

肺细胞在健康和疾病中的功能

• 批准号: 3337372
• 负责人: EUGENE D. ROBIN
• 金额: $ 26.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-05-01 至 1987-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3337372
• 关键词: alveolar macrophages; cell type; cellular pathology; enzyme linked immunosorbent assay; fibroblasts; gene expression; genetic library; genetic regulation; glycolysis; hyperoxia; hypoxia; immunochemistry; isozymes; lung; medical education; mitochondria; mitochondrial DNA; monoclonal antibody; phagocytosis; pulmonary fibrosis /granuloma; radioimmunoassay; radiotracer; respiratory function; ribosomal RNA; tissue /cell culture

The coordinate genetic regulation of glycolysis by hypoxia will be studied in cell systems by measurements of glycolytic enzyme content, rates of biosynthesis and degradation; measurements of levels of specific mRNA for the various glycolytic enzymes and measurements of the rates of biosynthesis and degradation of the specific mRNAs. A variety of cell models will be used. The regulation of genetic expression in mitochondria by hypoxia will be studied in isolated cell systems and organs by measurements of mt enzyme activity and content, measurements of mt DNA and mt 16S ribosomal RNA. Alterations in collagen metabolism in fibroblasts at a genetic level by hyperoxia, hypoxia, Bleomycin and phagocytes will be studied in various fibroblast systems. The possibility of coordinate genetic regulation of collagen biosynthesis will be studied by investigating alterations in specific mRNA for the 6 post-translational enzymes. In addition, the proposal will be used as a training format for medical students, pre-doctoral PhD candidates, post-doctoral MD fellows and post-doctoral PhD fellows through separate funding.

研究缺氧对糖酵解的协调遗传调控 通过测量细胞系统中的糖酵解酶含量、速率 生物合成和降解；特定 mRNA 水平的测量 各种糖酵解酶及其速率的测量 特定 mRNA 的生物合成和降解。 多种细胞 将使用模型。 缺氧对线粒体基因表达的调节 通过测量 mt 酶在分离的细胞系统和器官中进行研究 活性和含量，mt DNA 和 mt 16S 核糖体 RNA 的测量。 成纤维细胞中胶原蛋白代谢在基因水平上的改变 高氧、缺氧、博莱霉素和吞噬细胞将在不同的环境中进行研究 成纤维细胞系统。 胶原蛋白生物合成协调遗传调控的可能性 将通过调查 6 种特定 mRNA 的变化来进行研究 翻译后酶。 此外，该提案将用作医疗培训格式 学生、博士前博士研究生、博士后医学博士研究员和 博士后博士研究员通过单独的资助。