LUNG CELL FUNCTION IN HEALTH AND DISEASE

将研究低氧对糖酵解的协同遗传调控 在细胞系统中，通过测量糖酵解酶含量、 生物合成和降解;测量特定mRNA的水平， 各种糖酵解酶和测量的速度， 特定mRNA的生物合成和降解。 多种细胞 模型将被使用。 缺氧对线粒体基因表达的调控将是 通过测量mt酶在分离的细胞系统和器官中进行研究 活性和含量，mt DNA和mt 16 S核糖体RNA的测量。 在遗传水平上改变成纤维细胞胶原代谢 高氧、缺氧、博来霉素和吞噬细胞将在各种不同的环境中进行研究。 成纤维细胞系统 胶原生物合成协同遗传调控的可能性 将通过研究6个细胞的特异性mRNA的改变来研究。 翻译后酶 此外，该建议将被用作医疗培训形式， 学生，博士前博士候选人，博士后MD研究员和 通过单独的资金资助博士后博士研究员。

The coordinate genetic regulation of glycolysis by hypoxia will be studied in cell systems by measurements of glycolytic enzyme content, rates of biosynthesis and degradation; measurements of levels of specific mRNA for the various glycolytic enzymes and measurements of the rates of biosynthesis and degradation of the specific mRNAs. A variety of cell models will be used. The regulation of genetic expression in mitochondria by hypoxia will be studied in isolated cell systems and organs by measurements of mt enzyme activity and content, measurements of mt DNA and mt 16S ribosomal RNA. Alterations in collagen metabolism in fibroblasts at a genetic level by hyperoxia, hypoxia, Bleomycin and phagocytes will be studied in various fibroblast systems. The possibility of coordinate genetic regulation of collagen biosynthesis will be studied by investigating alterations in specific mRNA for the 6 post-translational enzymes. In addition, the proposal will be used as a training format for medical students, pre-doctoral PhD candidates, post-doctoral MD fellows and post-doctoral PhD fellows through separate funding.