MECHANISMS AND SITES OF VENTILATORY NEUROGENESIS

本报告所建议的研究活动有两个目的：1） 评价多个部位产生呼吸节律的能力 脑干内，2）评估神经生理学基础 延髓脊髓部分支持的排泄活动 中枢神经系统。 关于后者，我们认为， 球脊髓呼吸节律产生的检查将是 因为球脊髓元件代表了一个潜在的更简单的 呼吸控制器，根据定义，是解剖学上更简单的实体， 比整个脑桥-延髓脊髓复合体更复杂 实验将是 为实现上述两个目标而进行的活动将有一个共同的目标， 可逆性或永久性阻断神经活动， 脑桥延髓连接 因此，通过这里将要描述的过程， 呼吸模式可能会可逆地改变， 脑干和脊髓可逆地与其他部分分离， 中枢神经系统 呼吸调节活动在 “孤立的神经节”将通过记录活动进行监测， 周围神经和/或颅神经的活动和单个 自动调节脑干单位 我们相信研究结果 本文提出的建议将提供重要的见解的机制 负责自动排泄活动的神经发生。

The aims of the research activities proposed herein are twofold: 1) to evaluate the capacity for respiratory rhythm generation at multiple sites within the brainstem and, 2) to evaluate the neurophysiological underlying the ventilatory activity which may be supported by the bulbospinal portion of the central nervous system. In the context of the latter, we believe that examinations of bulbospinal respiratory rhythm generation will be fruitful since bulbospinal elements represent a potentially simpler respiratory controller and, by definition, an anatomically simpler entity than the entire pontile-bulbospinal complex. Experiments which will be conducted to fulfill the above two aims will have as one common objective, the reversible or permanent blockade of neural activity at the pontomedullary junction. Thus, by procedures to be described herein, respiratory patterns may be reversibly altered and segments of the brainstem and spinal cord reversibly isolated from other portions of the central nervous system. Respiratory-modulated activity within the "isolated neural segments" will be monitored by recording activity in peripheral and/or cranial nerves and activity of single respiratory-modulated brainstem units. We believe that results of studies proposed herein will provide significant insights into the mechanisms responsible for the neurogenesis of automatic ventilatory activity.