ELECTRON DENSITY MAPPING OF HEMOGLOBIN MODEL COMPOUNDS

精确的结晶学方法正在被用来绘制 血红蛋白模型化合物的电子分布 自旋态、价态和配位数的函数。这个 研究将集中在小分子(NO、CO、O2) 卟啉和钴-席夫碱的加合物来阐明 金属-加合物成键的电子性质对我们的 了解氧摄取机制。具体来说，我们 将得出从金属到配体的电荷转移的量， 金属配体相互作用的共价性与非球面性 金属原子的电荷分布。此信息是 有望填补我们现有知识的重要空白，并 回答一些与程度有关的具体问题 亚铁血红素电子结构的变化对 氧装载和卸载的协同作用机制。 这些结果将有助于我们理解大量的 涉及到血红素蛋白的生物过程。 实验数据也将在常规X射线上收集 作为同步加速器源。特别是，摇摆器光束线在 将使用康奈尔高能同步加速器源CHESS 以获得液氦温度下的数据。增加的 在低波长和极低波长下提供分辨率 温度构成了实验中的一个重大改进 方法：研究方法。

Accurate crystallographic methods are being used to map the electron distribution of hemoglobin model compounds as a function of spin state, valency and coordination number. The research is to be focussed on small molecule (NO, CO, O2) adducts of porphyrins and cobalt Schiff bases to elucidate the electron nature of metal-adduct bonding which is essential for our understanding of the oxygen uptake mechanism. Specifically we will derive the amount of charge transfer from metal to ligand, the covalency of the metal ligand interaction and the asphericity of the metal atom charge distribution. This information is expected to fill important gaps in our present knowledge and answer a number of specific questions related to the extent to which changes in electronic structure of the heme contribute to the cooperativity mechanism for loading and unloading of oxygen. The results will aid in our understanding of the large number of biological processes involving heme proteins. Experimental data will be collected on conventional X-ray as well as synchrotron sources. In particular, the wiggler beamline at CHESS, the Cornell High Energy Synchrotron Source, will be used to obtain data at liquid helium temperature. The increased resolution available at low wavelenghts combined with very low temperatures constitute a major improvement in experimental methods.