PAA ADSORPTION ON POLYMERS USING TIRF AND FTIR/ATR

使用 TIRF 和 FTIR/ATR 在聚合物上吸附 PAA

• 批准号: 3338986
• 负责人: CHANNING R ROBERTSON
• 金额: $ 5.79万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-04-01 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3338986
• 关键词: adsorption; antithrombogenic surface; biomaterial compatibility; biomaterial evaluation; biomaterial interface interaction; blood proteins; cardiovascular prosthesis; conformation; copolymer; fibrinogen; homopeptide; infrared spectrometry; polylysine; polymers; reflection spectrometry; silicone rubber

Adsorption of serum proteins from blood onto an artificial surface constitutes the initial event in the sequence of steps leading to thrombus formation. Understanding of the protein adsorption phenomenon is, therefore, essential to elucidating the mechanisms responsible for blood-surface compatibility. The chemical heterogeneity of native serum proteins, however, prevents precise elucidation of the mechanisms responsible for protein/surface interactions. Thus, the research program described herein focuses on studying the interactions of chemically simple polyaminoacids (PAA) and their copolymers with a variety of polymeric surfaces. Total internal reflection fluorescence (TIRF) has been shown to be a versatile tool for studying the quantitative aspects of protein adsorption. This will be used to investigate the adsorption and desorption of kinetics, as well as to determine the maximum surface coverage for each PAA/surface pair. Fourier transform infrared spectroscopy/attenuated total reflection (FTIR/ATR) is capable of detecting conformation changes that protein molecules undergo during and after adsorption, and will be used to examine these conformational aspects of PAA adsorption. The protein and PAA adsorption processes can be strongly influenced by the presence of a shear field. The extent to which mass transfer limitations affect PAA adsorption rates depends on the strength of the shear field. In addition, PAA molecules may be oriented or denatured by shear. Thus, the effect of shear fields on PAA adsorption will be a central concern of the proposed research. Specifically, the adsorption characteristics of poly-lysine, poly-leucine, poly-glutamic acid and poly-tyrosine and the copolymers on polydimethylsiloxane, polydiphenylsiloxane, polycyanopropylmethylsiloxane, polymethylmethacrylate, polystyrene-sulfonate, polyethyleneoxide, polyvinylpyrrolidone, and polyhydroxyethylmethacrylate in well-characterized flow systems will be investigated. The results of these studies are expected to provide fundamental insights into the bonding reactions characteristic of proteins interacting with polymer surfaces in known hydrodynamic environments.

人工表面吸附血液中血清蛋白的研究 构成了导致血栓形成的一系列步骤中的初始事件 阵 对蛋白质吸附现象的理解是， 因此，对于阐明负责 血液表面相容性 天然血清的化学异质性 然而，蛋白质阻止了对机制的精确阐明， 负责蛋白质/表面相互作用。 因此，研究计划 本文所述的方法集中于研究化学上简单的 聚氨基酸（PAA）及其与各种聚合物的共聚物 表面。 全内反射荧光（TIRF）已被证明是一种 研究蛋白质定量方面的通用工具 吸附作用 这将用于研究吸附和解吸 的动力学，以及确定最大的表面覆盖率为每 PAA/表面对。 傅里叶变换红外光谱/衰减全 反射（FTIR/ATR）能够检测构象变化， 蛋白质分子在吸附过程中和吸附后进行，并将用于 研究PAA吸附的这些构象方面。 蛋白质和PAA吸附过程可以受到以下因素的强烈影响： 剪切场的存在。 传质限制的程度 影响PAA吸附速率取决于剪切场的强度。 在 此外，PAA分子可以通过剪切取向或变性。 因此 剪切场对PAA吸附的影响将是本文研究的中心问题。 提议的研究。 具体而言，聚赖氨酸，聚亮氨酸， 聚谷氨酸和聚酪氨酸及其共聚物 聚二甲基硅氧烷，聚二苯基硅氧烷，聚氰基丙基甲基硅氧烷， 聚甲基丙烯酸甲酯，聚苯乙烯磺酸盐，聚环氧乙烷， 聚乙烯吡咯烷酮和聚甲基丙烯酸羟乙酯 将研究良好表征的流动系统。 的结果予以 这些研究有望提供有关 蛋白质与聚合物表面相互作用的反应特征， 已知的流体动力学环境。