REGULATION OF HEPATIC LYMPH LIPOPROTEINS

本课题的目的是研究载脂蛋白和脂质 肝淋巴脂蛋白的组合物，并且还表征 肝淋巴管插管大鼠体内脂蛋白研究 由肝脏分泌。 为了实现这些目标，我们将首先完成 表征肝淋巴液的载脂蛋白和脂质组成， 脂蛋白 然后，通过使用离体灌流的肝脏，但与 肝淋巴管插管，我们将测量的数量和类型， 分泌到淋巴和灌注液中的脂蛋白（即，血管 途径），已知其在正常和实验条件下修饰 肝VLDL生成。 我们还将测量等离子体的贡献 VLDL对肝淋巴中的VLDL通过使用维生素A标记 VLDL。 为了进一步验证使用肝淋巴管插管的有效性， 大鼠作为研究肝脏脂蛋白分泌的体内模型，我们 将研究肝淋巴VLDL中载脂蛋白和脂质的输出， 用高糖饮食、酒精、乙炔 雌二醇和4-氨基吡唑并嘧啶。 最后是肝淋巴 将使用插管大鼠来研究肠淋巴液的作用 转移肝脏的脂蛋白分泌。 远景目标 本研究的主要目的是使用这种体内模型来研究影响 调节肝脏脂蛋白的分泌和新陈代谢 肝淋巴脂蛋白。

The goals of this proposal are to study the apoprotein and the lipid compositions of hepatic lymph lipoproteins, and also to characterize the hepatic lymph cannulated rat as an in vivo model for studying lipoprotein secretion by the liver. To achieve these goals, we will first finish characterizing the apoprotein and lipid composition of hepatic lymph and its lipoproteins. Then, by using the isolated perfused liver but with the hepatic lymph duct cannulated, we will measure the quantity and the type of lipoproteins secreted into the lymph and the perfusate (i.e., the vascular pathway) under normal and experimental conditions which are known to modify hepatic VLDL production. We will also measure the contribution of plasma VLDL to the VLDL in hepatic lymph through the use of vitamin A labeled VLDL. To further test the validity of using the hepatic lymph cannulated rat as an in vivo model for studying lipoprotein secretion by the liver, we will study the output of apoprotein and lipid in hepatic lymph VLDL when the rats have been treated with a high sucrose diet, alcohol, ethynyl estradiol and 4-aminopyrazolopyrimidine. Lastly, the hepatic lymph cannulated rat will be used to study the effect of intestinal lymph diversion on lipoprotein secretion by the liver. The long-term objectives of this research are the use of this in vivo model to study the factors that regulate the secretion of lipoproteins by the liver and the metabolism of the hepatic lymph lipoproteins.