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INTERNAL MEMBRANE SYSTEM OF ISOLATED CARDIAC CELLS

离体心肌细胞的内膜系统

基本信息

批准号：
3343646
负责人：
LEE E MOORE
金额：
$ 10.1万
依托单位：
UNIVERSITY OF TEXAS MED BR GALVESTON
依托单位国家：
美国
项目类别：
财政年份：
1984
资助国家：
美国
起止时间：
1984-06-01 至 1988-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3343646
关键词：
heart cell heart electrical activity intracellular membranes muscle contraction passive transport sarcoplasmic reticulum striated muscles

项目摘要

The purpose of th is project is to determine the role which the transverse tubules and sarcoplasmic reticulum play in the basic electrophysiological properties of single heart cells. This will be done by determining the best equivalent circuit representation of an individual cell based on both electrical and morphological measurements. The standard current and voltage clamp electrphyciological methods will be used in conjuction with the classical impedance methods successfully used to do similar studies on skeletal muscle. The equivalent circuit analysis will be used to rigorously assess the validity of voltage clamp measurements of ionic currents. The voltage dependent conductance of sodium, potassium, and calcium ions will be measured with large and small signal step voltage clamps. The kinetic parameters of these two measurements will be directly compared with those obtained from the new technique of white noise analysis. The patch clamp will be used to compare the passive properties of the surface membrane with regions containing either dyadic structure or transverse tubules. Focal stimulation will be used to find low threshold regions for a contraction response analogous to earlier measurements done on skeletal muscle showing excitation-contraction coupling at the location of the transverse tubules.

该项目的目的是确定横向小管和肌浆网在基本的电生理活动中起着重要作用。单个心脏细胞的特性。这将通过确定最佳等效电路表示的单个细胞的基础上，电学和形态学测量。标准电流和电压钳电生理学方法将与经典的阻抗方法成功地用于进行类似的研究骨骼肌等效电路分析将用于严格评估离子电流的电压钳测量的有效性。的电压钠、钾和钙离子的依赖电导将是用大信号和小信号阶跃电压箝位测量。动力学这两个测量的参数将直接与那些从白色噪声分析的新技术获得。膜片钳将用于比较表面膜的区域含有二元结构或横小管局灶刺激将用于寻找低阈值收缩反应的区域类似于早期的测量结果，在骨骼肌上显示兴奋-收缩偶联，横小管的一部分