DETECTION OF THE CARRIER STATE OF AIDS

艾滋病可通过输血传播的证据 艾滋病高危人群的无症状成员促使人们寻找一种 一种筛查测试，可用于识别 无序。我们建议对精选的替代检测方法进行评估 预测艾滋病或持续性淋巴结病发展的能力 一组罹患疾病风险增加的同性恋者的综合症 这些障碍。我们还将研究两组志愿献血者 实验室检查结果在我们的普通捐献者库中很少见，但是 已知在艾滋病和艾滋病患者中更为普遍 相关障碍。将在三年内连续研究的种群将 包括150名看起来很健康男同性恋者 性伴侣，100名自愿献血者持续倒置 辅助性/诱导性T淋巴细胞与抑制性/细胞毒性T淋巴细胞的比值(Th：TS)， 100名人类T细胞抗体阳性的志愿献血者 白血病/淋巴瘤病毒(HTLV)和50名Th：TS自愿献血者 在正常范围内。5名完全表达艾滋病的患者分组 50名患有持续性全身性淋巴结病的患者将在 仅限于研究的开始。除了临床随访外， 以下化验将在研究开始时和 此后6个月的间隔：使用A/D分析T淋巴细胞亚群 一组单抗，包括几种新试剂； 血清中可溶性淋巴细胞表面分子的测定； 检测三种已识别的人类T细胞抗体的试验 白血病淋巴瘤病毒；循环免疫复合物试验； 血清β-2-微球蛋白和胸腺素α-1的水平；以及 乙肝核心抗原(HBcAb)抗体的存在。这些化验 在鼓励初步研究的基础上被选为研究对象 以及潜在的方法论简便性。临床与临床的相关性分析 艾滋病高危人群和献血人群的实验室发现可能会有所帮助 确定最能预测的程序或程序组合 艾滋病或相关疾病的后续发展，有可能 应用程序筛查捐献的血液以检测艾滋病携带者状态。

Evidence that AIDS may be transmitted by transfusion of blood from asymptomatic members of AIDS high risk groups has prompted a search for a screening test that can be used to identify a carrier state for the disorder. We propose to assess selected alternative assays for their ability to predict the development of AIDS or persistent lymphadenopathy syndrome in a group of homosexual men at increased risk for developing these disorders. We will also study two groups of volunteer blood donors with laboratory findings seen uncommonly in our general donor pool, but known to be significantly more prevalent in patients with AIDS and AIDS related disorder. The populations to be studied serially over 3 years will include 150 apparently healthy homosexual men with multiple anonymous sexual partners, 100 volunteer blood donors with persistent inversions of the ratio of helper/inducer to suppressor/cytotoxic T lymphocytes (Th:Ts), 100 volunteer blood donors with antibodies to the human T cell leukemia/lymphoma viruses (HTLV), and 50 volunteer blood donors with Th:Ts within the normal range. Groups of 5 patients with fully expressed AIDS and 50 with persistent generalized lymphadenopathy will be tested at the beginning of the study only. In addition to clinical follow-up, the following assays will be performed at the initiation of the study and at six month intervals thereafter: analysis of T lymphocyte subsets using a panel of monoclonal antibodies, including several novel reagents; measurement in serum of soluble forms of lympohcyte surface molecules; tests to detect antibodies to the three identified types of human T cell leukemia lymphoma viruses (HTLV); tests of circulating immune complexes; serum levels of beta-2-microglobulin and thymosin alpha-1; and tests for the presence of antibody to hepatitis B core antigen (HBcAb). These assays have been chosen for study on the basis of encouraging preliminary studies and potential ease of methodology. Correlation of the clinical and laboratory findings in the AIDS hight risk and blood donor groups may help to identify the procedure or combination of procedures best able to predict subsequent development of AIDS or related disorders, with potential applications to screening donated blood to detect an AIDS carrier state.