NERVOUS AND NEUROHUMORAL INFLUENCES ON CARDIAC MYOCYTES

本研究的主要目的是了解 在细胞和分子水平上控制生长的机制 分离的成人心肌细胞。 这项研究将在 利用仔细定义的条件（即无血清）的组织培养 媒体）和分子生物学的最新进展（原位杂交和 使用α和β特异性探针的S1核酸酶保护实验， α和β异肌球蛋白的单克隆抗体），以检查 神经和神经体液对肌细胞分化的影响。 我们将 研究收缩蛋白的合成，特别是肌球蛋白和 在分子水平上调节这种合成。 另夕h 神经和神经体液影响与心肌细胞的关系 将研究扩散问题。 组织培养的使用将允许 分析具体的变量，而不是复杂的系统 相互作用通常会影响体内的解释 实验 这项研究的成功完成将 允许更清楚地了解那些控制心肌细胞的因素 生长和适应压力。

The major aim of this research is to gain an understanding of the mechanisms which at the cellular and molecular level control the growth of the isolated adult cardiac myocyte. This research will be undertaken in tissue culture utilizing carefully defined conditions (i.e. serum-free media) and recent advances in molecular biology (In situ hybridization and S1 nuclease protection experiments with Alpha and Beta specific probes, monoclonal antibodies to Alpha and Beta isomyosins) to examine the effects of neural and neurohumoral influences on myocyte differentiation. We will examine the synthesis of contractile proteins, in particular myosin and the regulation of this synthesis at the molecular level. Additionally, the relationship between neural and neurohumoral influences and myocyte proliferation will be studied. The use of tissue culture will permit the analysis of specific variables as opposed to the complicated systemic interactions which usually affect the interpretation of in vivo experiments. The successful completion of this proposed research will permit a clearer insight into those factors controlling cardiac myocyte growth and adaptation to stress.