FUNCTIONAL ANALYSIS OF VASCULAR ANGIOTENSIN II

本次调查的目的是确定功能 血管系统中产生的血管紧张素II（AII）对 体循环动脉压（BP）、基础血管张力和 肾上腺素能反应 负责释放肾素的刺激 和来自完整血管床的所有II将被研究。 因为 其高反应性的肾素-血管紧张素系统和稳定的 心血管系统在抽取血液样本的过程中 如果需要进行试验，将使用家兔。 股动脉 将在戊巴比妥中监测灌注压和BP 麻醉的兔子 股静脉分流术可以 在不进行再循环的情况下，向后肢施用卡托普利 从而允许选择性血管紧张素转化酶 抑制作用 肾切除和假手术家兔将 用于比较AII形成的相对重要性 后肢血管系统的基础参数。 为了消除 肾切除术后并发症对电解质平衡和血浆的影响 尿素，并进一步诱导血管AII的影响， 动物将在每次实验前进行腹膜透析， 术后1、2和4天，研究血管介入治疗 肾素和AII的释放将是β 1和β 2肾上腺素受体 刺激，交感神经的直接电刺激， 出血、传入神经刺激、钙变化和 钠和高血压。 伴随而来的变化 血压、股动脉血管阻力和股动脉血管对 肾上腺神经刺激和去甲肾上腺素 测定 开托普利和萨拉辛将被用作一种手段 区分血管中的肾素 血管紧张素系统在这些干预。 因为 血管AII在血压调节中的假定作用， 在疾病状态如高血压和 充血性心力衰竭和可能在基础条件下， 了解这个系统很重要。

The purpose of this investigation is to determine the functional role of angiotensin II (AII) generated in the vasculature on systemic arterial pressure (BP), basal vascular tone and adrenergic reactivity. Stimuli responsible for the release of renin and AII from the intact vascular bed will be studied. Because of its highly reactive renin-angiotensin system and stable cardiovascular system during withdrawal of blood samples for the required assays, the rabbit will be utilized. Femoral artery perfusion pressure and BP will be monitored in pentobarbital anesthetized rabbits. A femoral venous shunt will allow for administration of captopril to the hindlimb without recirculating and thus allow for selective angiotensin converting enzyme inhibition. Nephrectomized and sham operated rabbits will be employed to compare the relative importance of AII formed in the hindlimb vasculature on basal parameters. In order to eliminate complications of nephrectomy on electrolyte balance and plasma urea and to further induce the influence of vasculature AII, the animals will undergo peritoneal dialysis before each experiment at 1, 2 and 4 days post op. Interventions to be studied on vascular renin and AII release will be beta 1 and beta 2-adrenoceptor stimulation, direct electrical stimulation of sympathetic nerves, hemorrhage, afferent nerve stimulation, changes in calcium and sodium and Goldblatt hypertension. The accompanying changes in BP, femoral vascular resistence and femoral vascular responses to adrengeric nerve stimulation and norepinehrine will be determined. Captopril and saralasin will be employed as a means of distinguishing the involvement of the vascular renin- angiotensin system during these intervetnions. Because of the postulated role of vascular AII in the regulation of BP and vascular tone in disease states such as hypertension and congestive heart failure and possibly under basal conditions, knowledge about this system is important.