EFFECTS OF OMEGA-3 FATTY ACIDS ON LIPID TRANSPORT

OMEGA-3 脂肪酸对脂质运输的影响

• 批准号: 3355951
• 负责人: ANDRE BENSADOUN
• 金额: $ 9.32万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1992-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3355951
• 关键词: adipocytes; antihyperlipoproteinemic agent; binding proteins; blood lipid; blood lipoprotein; blood lipoprotein metabolism; chickens; cholesterol; chylomicrons; diet therapy; enzyme mechanism; essential fatty acids; fatty acid metabolism; fatty acid transport; glycoproteins; heparan sulfate; lipid transport; lipoprotein lipase; liver cells; liver function; low density lipoprotein; marine animal oil; nutrition related tag; omega 3 fatty acid; radioimmunoassay; tissue /cell culture; triglycerides; vascular endothelium; vegetable oils; very low density lipoprotein

The objective of the proposal is to understand the mechanisms whereby dietary w3 fatty acids when substituted for w6 fatty acids lower plasma lipids. The central hypothesis is that w3 fatty acids when substituted for w6 fatty acids in membranes enhance the activity of lipoprotein lipase (LPL) and hepatic lipase (HL). Because of the enhanced peripheral delivery of chylomicron and VLDL fatty acids and cholesterol. The influx of these constituents to the liver is deceased. The decreased hepatic input would account for the deceased VLDL production. An increased hepatic lipase activity with w3 fatty acid feeding would enhance the uptake of chylomicron and VLDL remnant. Finally it is hypothesized that enrichment of w3 fatty acids in hepatic membranes enhances LDL receptor activity. VLDL secretion rates, VLDL to LDL fluxes, and fractional removal rates will be measured in roosters fed 0.5% cholesterol and either 10% fish oil or 10% corn oil. VLDL protein and lipid secretion rates by hepatocytes prepared from animals fed the experimental diets will be compared. LPL synthesis, degradation, and secretion by adipocytes incubated with w3 or w6 fatty acids will be determined. The effect of w3 series on HL synthesis, degradation, and secretion will also be determined in hepatocytes in culture. Binding constants of lipolytic enzymes for endothelial cells will be measured. The fractional removal rate and the sites of degradation of LDL and IDL by receptor and receptor independent pathways will be determined with the two diets. The fractional removal rate of (14C) sucrose IDL and (14C) sucrose methylated IDL will be determined in animals where LPL is inhibited by antiserum to prevent conversion of IDL to LDL. Recycling of plasma membrane sialoglycoproteins and heparan sulfate will be determined in cells enriched with w6 or w3 fatty acids. Finally hepatic LDL receptor activity will be assessed by binding studies of (123I) LDL, IDL, and chylomicron remnants to liver membranes.

该提案的目的是了解 由此当用膳食W3脂肪酸代替W6脂肪酸时， 酸降低血浆脂质。 核心假设是w3脂肪 当在膜中取代W6脂肪酸时， 脂蛋白脂酶（LPL）和肝脂酶（HL）活性。 由于乳糜微粒的外周输送增强， VLDL脂肪酸和胆固醇。 这些人的涌入 肝脏的成分已经死亡。 肝输入减少 会导致VLDL的产生 增加 饲喂W3脂肪酸可提高肝脂酶活性， 乳糜微粒和VLDL残留的摄取。 最后是 假设肝脏中W3脂肪酸富集 膜增强LDL受体活性。 VLDL分泌 速率，VLDL到LDL通量，以及分数去除率将是 在喂食0.5%胆固醇和10%鱼油的公鸡中测量 或者10%的玉米油 VLDL蛋白和脂质分泌率， 从喂食实验饲料的动物中制备的肝细胞 将被比较。 LPL的合成、降解和分泌 用W3或W6脂肪酸孵育的脂肪细胞将 测定 w3系列对HL合成的影响， 还将在肝细胞中测定降解和分泌 在文化中。 脂解酶与内皮细胞的结合常数 细胞将被测量。 部分去除率和部位 LDL和IDL降解的受体和受体 将用两种饮食确定独立的途径。 的 （14 C）蔗糖IDL和（14 C）蔗糖的部分去除率 将在LPL为 被抗血清抑制以防止IDL转化为LDL。 质膜唾液酸糖蛋白和乙酰肝素的再循环 将在富含W6或W3脂肪酸的细胞中测定硫酸盐 acids. 最后，将通过以下方法评估肝脏LDL受体活性： （123 I）LDL、IDL和乳糜微粒残留物与 肝膜

项目成果

Lipoprotein lipase synthesis and secretion: effects of concentration and type of fatty acids in adipocyte cell culture.

脂蛋白脂肪酶的合成和分泌：脂肪细胞培养物中脂肪酸浓度和类型的影响。

• 发表时间: 1993
• 期刊: Journal of lipid research
• 影响因子: 6.5
• 作者: Montalto,MB;Bensadoun,A
• 通讯作者: Bensadoun,A