MOLECULAR STUDIES ON OPOID PEPTIDES AND PROCESSING ENZYM

本申请是申请建立神经肽的部分资金。 俄勒冈高等生物医学研究所实验室 健康科学大学并为基础设施提供助学金支持 阿片肽及其加工酶的神经生物学研究。 请拨这些资金以使首席调查员能够继续 并延长他在担任南希成员时就开始的工作 斯坦福医学中心行为神经化学普利兹克实验室。 建议的研究包括四个部分： 1.进一步的生化和免疫组织化学表征 前强啡肽神经元系统。 2.进一步的生化和免疫组织化学表征 脑啡肽原神经元系统。 3.2个可能的蛋白降解过程的分离和鉴定 参与多肽激素前体成熟的酶。 4.新型光反应性阿片肽的合成与表征 它可以与特定的抗体一起用作免疫学 阿片受体的探头。 我们在这些研究项目中采用的方法包括： 多肽合成、抗体生产、放射免疫分析、层析 高效液相和亲和层析等技术，氨基酸 组成分析和气相氨基酸序列、多肽和 蛋白质分离、放射受体分析、免疫组织化学以及 蛋白质和多肽化学及生物化学的一般技术。 内源性阿片类药物是机体重要的神经肽系统之一。 哺乳动物有机体。我们试图回答的问题将是， 因此，要与一般的精神和神经学问题相关 通过研究生物化学和解剖学的基本原理来了解疾病 阿片肽、它们的受体及其加工酶。

This application is a request for partial funds to establish a neuropeptide laboratory at the Institute for Advanced Biomedical Research at the Oregon Health Sciences University and to provide grant support for basic neurobiological studies on opioid peptides and their processing enzymes. These funds are requested to enable the Principal Investigator to continue and extend the work he has begun while he was a member of the Nancy Pritzker Laboratory of Behavioral Neurochemistry at Stanford Medical Center. The research that is proposed consists of four sections: 1. Further biochemical and immunohistochemical characterization of the prodynorphin neuronal system. 2. Further biochemical and immunohistochemical characterization of the proenkephalin neuronal system. 3. Isolation and characterization of 2 putative proteolytic processing enzymes involved in peptide hormone precursor maturation. 4. Synthesis and characterization of novel photoreactive opioid peptides that can be used--in conjunction with specific antibodies--as immunological probes for opioid receptors. The methods that we are applying in these research projects include: peptide synthesis, antibody production, radioimmunoassay, chromatography techniques including HPLC and affinity chromatography, amino acid composition analysis and gas phase amino acid sequencing, peptide and protein isolation, radioreceptor assays, immunohistochemistry, as well as general techniques in protein and peptide chemistry and biochemistry. Endogenous opioids constitute one of the major neuropeptide systems in the mammalian organism. The questions we are attempting to answer will, therefore, be relevant to general problems of mental and neurological disorders by investigating basic principles of the biochemistry and anatomy of opioid peptides, their receptors and their processing enzymes.