MOLECULAR STUDIES ON OPOID PEPTIDES AND PROCESSING ENZYM

这个申请是一个部分资金的请求，以建立一个神经肽 俄勒冈州高级生物医学研究所的实验室 健康科学大学，并提供赠款支持的基本 阿片肽及其加工酶的神经生物学研究。 请拨这些资金是为了使首席调查员能够继续 并将他作为南希的一员所做的工作继续下去 斯坦福大学医学中心普利兹克行为神经化学实验室。 拟议的研究包括四个部分： 1.进一步的生物化学和免疫组织化学表征， 前强啡肽神经元系统 2.进一步的生物化学和免疫组织化学表征， 前脑啡肽神经元系统 3. 2种蛋白水解酶的分离和鉴定 参与肽激素前体成熟的酶。 4.新型光反应性阿片肽的合成与表征 它可以与特异性抗体结合， 阿片受体的探针 我们在这些研究项目中采用的方法包括： 肽合成，抗体生产，放射免疫分析，色谱法 技术，包括HPLC和亲和层析，氨基酸 组成分析和气相氨基酸测序，肽和 蛋白质分离、放射受体测定、免疫组织化学，以及 蛋白质和肽化学和生物化学的一般技术。 内源性阿片样物质构成了中枢神经系统中的主要神经肽系统之一。 哺乳动物有机体。 我们试图回答的问题， 因此，与一般的精神和神经问题有关， 通过研究生物化学和解剖学的基本原理， 阿片肽及其受体和加工酶。

This application is a request for partial funds to establish a neuropeptide laboratory at the Institute for Advanced Biomedical Research at the Oregon Health Sciences University and to provide grant support for basic neurobiological studies on opioid peptides and their processing enzymes. These funds are requested to enable the Principal Investigator to continue and extend the work he has begun while he was a member of the Nancy Pritzker Laboratory of Behavioral Neurochemistry at Stanford Medical Center. The research that is proposed consists of four sections: 1. Further biochemical and immunohistochemical characterization of the prodynorphin neuronal system. 2. Further biochemical and immunohistochemical characterization of the proenkephalin neuronal system. 3. Isolation and characterization of 2 putative proteolytic processing enzymes involved in peptide hormone precursor maturation. 4. Synthesis and characterization of novel photoreactive opioid peptides that can be used--in conjunction with specific antibodies--as immunological probes for opioid receptors. The methods that we are applying in these research projects include: peptide synthesis, antibody production, radioimmunoassay, chromatography techniques including HPLC and affinity chromatography, amino acid composition analysis and gas phase amino acid sequencing, peptide and protein isolation, radioreceptor assays, immunohistochemistry, as well as general techniques in protein and peptide chemistry and biochemistry. Endogenous opioids constitute one of the major neuropeptide systems in the mammalian organism. The questions we are attempting to answer will, therefore, be relevant to general problems of mental and neurological disorders by investigating basic principles of the biochemistry and anatomy of opioid peptides, their receptors and their processing enzymes.