SYNAPTIC PLASTICITY IN THE SYMPATHETIC NERVOUS SYSTEM

拟议调查的目的是确定以下原则 控制突触的特异性和可塑性。颈上交感神经 大鼠神经节是被选择用于研究的系统，因为它提供了 比较突触前和突触后相互作用的机会 在几种不同条件下的神经元。这些情况是正常的 节前去神经后的发育、再生、侧枝萌发 通过节前去神经和突触切除后的外来轴突 神经节后轴索切断术后的修复。 神经节将主要通过定量电子显微镜进行分析， 特别强调突触前终末和突触之间的相互作用 突触后膜在突触发生过程中特化。此外，a 拟议研究的一部分将涉及制定有效的 神经节细胞超微结构定位的可靠方法 胆碱能受体及其在突触发生中的作用。 拟议调查获得的结果将有助于 理解突触前和突触后神经元施加的限制 在正常发育和损伤后的突触形成过程中 对神经系统的影响。

The purpose of the proposed investigation is to determine the principles which govern synaptic specificity and plasticity. The superior cervical sympathetic ganglion of the rat is the system chosen for study, since it provides the opportunity to compare the interactions between the pre- and postsynaptic neurons under several different conditions. These conditions are normal development, regeneration after preganglionic denervation, collateral sprouting by foreign axons after preganglionic denervation, and synapse removal and restoration after postganglionic axotomy. The ganglia will be analyzed primarily by quantitative electron microscopy with particular emphasis on the interactions between the presynaptic terminals and the postsynaptic membrane specializations during synaptogenesis. In addition, a portion of the proposed study will be concerned with the development of valid and reliable methods for the ultrastructural localization of the ganglionic cholinergic receptors and their role in synaptogenesis. The results obtained from the proposed investigation will contribute toward understanding the constraints imposed by the pre- and postsynaptic neurons during the synapse formation which occurs in normal development and after injury to the nervous system.