STATE DEPENDENT CONTROL OF SOMATIC REFLEX ACTIVITY

躯体反射活动的状态依赖性控制

• 批准号: 3394126
• 负责人: MICHAEL H CHASE
• 金额: $ 17.73万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-08-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3394126
• 关键词: adrenergic agents; antiadrenergic agents; brain electrical activity; brain stem; electrophysiology; experimental brain lesion; medulla oblongata; membrane potentials; microelectrodes; microinjections; motor neurons; neural information processing; neural inhibition; neural initiation; neuropharmacology; neuropsychology; neurotransmitters; nontherapeutic iontophoresis; pons; psychobiology; psychophysiology; reticular formation; sensorimotor system; sleep; somatic reflex; synapses; wakefulness

The research described in this application is designed to examine the neurotransmitters and neuronal circuitry which are responsible for the state-dependent modulation of trigeminal motoneuron membrane potential activity during sleep and wakefulness. Interdisciplinary electrophysiological and iontophoretic studies are proposed, each of which relates to and complements the others. These studies, which are organized within the framework of the examination of the paradoxical phenomenon of "reticular response-reversal", entail a determination of the state-dependent activity of the interrelationships between the pontine nucleus pontis oralis, the medullary nucleus reticularis gigantocellularis and the trigeminal motor nucleus. The proposed studies will (1) provide a comprehensive description of the frequency of occurrence and a quantitative analysis of the waveform characteristics of the newly-discovered inhibitory and excitatory postsynaptic potentials that are involved in the modulation of brain stem motoneuron membrane potential activity during sleep and wakefulness, (2) generate a normative fund of information relating to the inhibitory and excitatory neurotransmitters which mediate the postsynaptic inhibition and excitation of brain stem motoneurons during these states, and (3) establish a prototypical experimental paradigm for utilizing spike- triggered averaging, intracellular recording and iontophoretic techniques to study the cellular bases of behavior in the chronic, unanesthetized, normally respiring animal.

本申请中描述的研究旨在检查 神经递质和神经回路是 三叉神经的状态依赖性调制 睡眠和睡眠时运动神经元的膜电位活动 保持清醒。跨学科电生理学和 提出了离子电泳学研究，每一项研究都与和 与其他的相辅相成。这些研究是有组织的 在考察悖论的框架内 “网状反应-反转”现象，需要一个 的状态依赖活性的测定 桥脑口侧核、脑桥核间的相互关系 巨细胞网状延髓核与三叉神经 运动核团。 拟议的研究将(1)提供一个全面的描述 出现的频率和数量的分析 新发现的抑制性和阻滞性的波形特征 兴奋性突触后电位参与 脑干运动神经元膜电位的调节 睡眠和清醒时的活动，(2)产生正常的 与抑制性和兴奋性有关的信息基金 介导突触后抑制的神经递质和 脑干运动神经元在这些状态下的兴奋，以及(3) 建立利用SPEKE的原型实验范式 触发平均、细胞内记录和离子导入 研究慢性病患者行为的细胞基础的技术， 未麻醉的、正常呼吸的动物。