MOLECULAR BASIS OF CELL RECOGNITION IN DEVELOPMENT

这项工作的主要长期目标是了解 控制重要神经元特性的表达 哺乳动物中枢神经系统的发育。 我们计划使用 单克隆抗体（MAbs），识别神经元的子集， 确定细胞特异性抗原何时首次表达。 特别是， 我们希望发现细胞特异性抗原表达是否发生在分裂过程中， 干细胞是成熟神经元的祖细胞，或者如果最终的神经元是干细胞， 有丝分裂必须在细胞特异性抗原表达开始之前发生。 我们 我也想知道是否形成适当的连接是 为了启动或维持细胞的表达， 特异性抗原 建议的一部分是使用两个新的 发现MABS RB-8染色大鼠嗅觉感觉细胞的亚群， 它们的轴突 我们将研究RB-8染色在 胚胎和成体嗅球。 在成年人中，我们将绘制 RB-8阳性纤维在整个球范围内的分布。 我们将在几只成年大鼠身上做这件事，看看这种模式是否不变 或者是否因动物而异。 RB-8染色的进展 将在胚胎和年轻动物中仔细观察。 最后 再生嗅觉投射中RB-8染色的模式将是 测定 RB-6之所以被选中，是因为在视网膜中，它只染色 视网膜神经节细胞 在RB-6的情况下，我们将观察外观 视网膜抗原阳性细胞在出生前，期间和之后， 神经节细胞 RB-6抗原在体外的表达也将被证实。 研究以确定靶细胞的存在是否影响抗原 表情 识别细胞表面抗原的单克隆抗体 神经元将在这一领域的未来研究中发挥重要作用。 的一部分 将努力选择新的单克隆抗体， 这些属性。 我们将使用神经肿瘤和膜， 开发大脑以免疫小鼠并产生额外的杂交瘤。 所有 工作将使用大鼠细胞和膜进行， 似乎是中枢神经系统生物学研究最深入的脊椎动物 很担心

The major long range goal of this work is to understand the mechanisms that control the expression of important neuronal properties during the development of the mammalian central nervous system. We plan to use monoclonal antibodies (MAbs) which recognize subsets of neurons to determine when cell specific antigens are first expressed. In particular, we wish to find out if cell specific antigen expression occurs in dividing stem cells that are the progenitors of the mature neuron or if the final mitosis must occur before cell specific antigen expression begins. We would also like to find out if the formation of appropriate connections is necessary in order to either initiate or maintain expression of cell specific antigens. One part of the proposal is to investigate these questions using two newly discovered MAbs. RB-8 stains a subset of rat olfactory sensory cells and their axons. We will study the distribution of RB-8 staining in the embryonic and adult olfactory bulb. In the adult we will map the distribution of RB-8 positive fibers over the entire extent of the bulb. We will do this in several adult rats to see if this pattern is invariant or if it varies from animal to animal. The development of RB-8 staining will be carefully followed in embryonic and young animals. Finally, the pattern of RB-8 staining in regenerating olfactory projections will be determined. RB-6 was seleted because, in the retina, it stains only the retinal ganglion cells. In the case of RB-6 we will observe the appearance of antigen positive cells in retinas before, during and after the birth of ganglion cells. The expression of RB-6 antigen in vitro will also be studied to determine if the presence of target cells influences antigen expression. Monoclonal antibodies which recognize cell surface antigens on subsets of neurons will prove important in future research in this area. Part of our efforts will be directed towards selecting new monoclonal antibodies with these attributes. We will use both neural tumors and membranes from the developing brain to immunize mice and produce additional hybridomas. All work will be carried out using rat cells and membranes as the rat now appears to be the most intensively studied vertebrate as far as CNS biology is concerned.