GLUTAMIC DEHYDROGENASE IN NEUROLOGIC DISORDERS
GLUTAMIC DEHYDROGENASE IN NEUROLOGIC DISORDERS
批准号:
3397201
负责人:
ANDREAS PLAITAKIS
金额:
$15.29万
依托单位国家:
美国
项目类别:
财政年份:
1987
资助国家:
美国
项目状态:
已结题
起止时间:
1987-09-07 至 1990-08-31
关键词:
Parkinson's disease aminoacid metabolism blood chemistry brain disorder diagnosis brain mapping cerebellar disorders electrofocusing enzyme mechanism enzyme structure fibroblasts gene expression genetic translation glutamate dehydrogenase human subject isoleucine laboratory rat leucine leukocytes posttranslational modifications protein biosynthesis tissue /cell culture
中文摘要
我们实验室最近的研究表明,
谷氨酸脱氢酶存在于哺乳动物的大脑中,
这种酶的异常发生在神经和
迟发性神经元性脑病患者的神经组织
退行性疾病 本提案的长期目标
是双重的:第一是进一步表征不同的
GDH组分,并确定它们是否代表
基因决定的变体或翻译后产物
改性 第二个是定义精确的分子
导致酶活性改变的缺陷
在神经病患者身上发现的。
我们建议:1)进一步研究动力学和电泳
纯化的GDH形式的特征,
患者和对照的神经组织。 2)搜索
在酶的一级结构的差异在健康和
疾病 3)研究培养皮肤合成GDH的能力
成纤维细胞的患者和对照,并调查是否后
酶分子的翻译修饰发生在
人体组织,如果是这样的话,这是否是改变了在
疾病状态。 4)为了确定细胞的亚细胞定位,
大脑中的酶,以便更好地了解其在神经系统中的作用。
系统功能和功能障碍。 这些研究预计将
为克隆人GDH基因奠定基础,
探索其在人类退行性疾病中可能的多态性
紊乱
英文摘要
Recent studies in our laboratory have shown that distinct forms
of glutamate dehydrogenase are present in mammalian brain and
that abnormalities of this enzyme occur in neural and
extraneural tissues of patients with late onset neuro-
degenerative disorders. The long-term objects of this proposal
are twofold: The first is to further characterize the different
GDH components and determine as to whether they represent
genetically determined variants or products of post-translational
modification. The second is to define the precise molecular
defect(s) that are responsible for the altered enzyme activity
found in the neurological patients.
We propose: 1) to study further the kinetic and electrophoretic
characteristics of the purified GDH forms from extraneural and
neural tissues of patients and controls. 2) To search for
differences in the primary structure of the enzyme in health and
disease. 3) To study the synthesis of GDH by cultured skin
fibroblasts of patients and controls and investigate whether post-
translational modification(s) of the enzyme molecule occurs in
human tissues, and if so as to whether this is altered in the
disease state. 4) To determine the subcellular localization of the
enzyme in brain in order to better understand its role in nervous
system function and dysfunction. These studies are expected to
lay the groundwork for cloning the human GDH gene(s) and
exploring its possible polymorphism in human degeneratives
disorders.
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GLUTAMIC DEHYDROGENASE IN NEUROLOGIC DISORDERS
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批准号:3397197
-
项目类别:
-
资助金额:$16.92万
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财政年份:1987
-
负责人:ANDREAS PLAITAKIS
-
依托单位:
GLUTAMIC DEHYDROGENASE IN NEUROLOGIC DISORDERS
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批准号:3397205
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项目类别:
-
资助金额:$28.14万
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财政年份:1987
-
负责人:ANDREAS PLAITAKIS
-
依托单位:
GLUTAMIC DEHYDROGENASE IN NEUROLOGIC DISORDERS
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批准号:3397204
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项目类别:
-
资助金额:$26.11万
-
财政年份:1987
-
负责人:ANDREAS PLAITAKIS
-
依托单位:
GLUTAMIC DEHYDROGENASE IN NEUROLOGIC DISORDERS
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批准号:3397203
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项目类别:
-
资助金额:$24.0万
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财政年份:1987
-
负责人:ANDREAS PLAITAKIS
-
依托单位:
GLUTAMIC DEHYDROGENASE IN NEUROLOGIC DISORDERS
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批准号:2263100
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项目类别:
-
资助金额:$31.65万
-
财政年份:1987
-
负责人:ANDREAS PLAITAKIS
-
依托单位:
GLUTAMIC DEHYDROGENASE IN NEUROLOGIC DISORDERS
-
批准号:3397202
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项目类别:
-
资助金额:$14.84万
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财政年份:1987
-
负责人:ANDREAS PLAITAKIS
-
依托单位:
GLUTAMIC DEHYDROGENASE IN NEUROLOGIC DISORDERS
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批准号:3397198
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项目类别:
-
资助金额:$25.38万
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财政年份:1987
-
负责人:ANDREAS PLAITAKIS
-
依托单位:
GLUTAMIC DEHYDROGENASE IN NEUROLOGIC DISORDERS
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批准号:3397199
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项目类别:
-
资助金额:$13.78万
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财政年份:1980
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负责人:ANDREAS PLAITAKIS
-
依托单位:
GLUTAMIC DEHYDROGENASE IN NEUROLOGIC DISORDERS
-
批准号:3397200
-
项目类别:
-
资助金额:$14.34万
-
财政年份:1980
-
负责人:ANDREAS PLAITAKIS
-
依托单位:
GLUTAMIC DEHYDROGENASE IN NEUROLOGIC DISORDERS
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批准号:3884362
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:ANDREAS PLAITAKIS
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依托单位:
GLUTAMIC DEHYDROGENASE IN NEUROLOGIC DISORDERS
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批准号:3741006
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:ANDREAS PLAITAKIS
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依托单位:
L-5 HYDROXYTRYPTOPHAN AND CARBIDOPA TRIAL IN ATAXIA
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批准号:3972602
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:ANDREAS PLAITAKIS
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依托单位:
GLUTAMIC DEHYDROGENASE IN NEUROLOGIC DISORDERS
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批准号:4700193
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:ANDREAS PLAITAKIS
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依托单位:
L-5 HYDROXYTRYPTOPHAN AND CARBIDOPA TRIAL IN ATAXIA
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批准号:4700203
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:ANDREAS PLAITAKIS
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依托单位:
GLUTAMATE AND ASPARTATE METABOLISM IN NEUROLOGIC DISEASE
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批准号:4697236
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:ANDREAS PLAITAKIS
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依托单位:
GLUTAMATE DEHYDROGENASE DYSFUNCTION IN BASAL GANGLIA DISORDERS
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批准号:3969334
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:ANDREAS PLAITAKIS
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依托单位:
GLUTAMIC DEHYDROGENASE IN NEUROLOGIC DISORDERS
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批准号:3947832
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:ANDREAS PLAITAKIS
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依托单位:
GLUTAMIC DEHYDROGENASE IN NEUROLOGIC DISORDERS
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批准号:3972592
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:ANDREAS PLAITAKIS
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依托单位:
GLUTAMATE DEHYDROGENASE DYSFUNCTION IN BASAL GANGLIA DISORDERS
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批准号:3945565
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:ANDREAS PLAITAKIS
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依托单位:
CONTROLLED TRIAL OF BRANCHED CHAIN MINO ACIDS IN ALS
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批准号:3904009
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:ANDREAS PLAITAKIS
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依托单位: