PURIFICATION AND CHARACTERIZATION OF ADENYLATE CYCLASE
PURIFICATION AND CHARACTERIZATION OF ADENYLATE CYCLASE
批准号:
3400844
负责人:
DANIEL R STORM
金额:
$12.24万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1984
资助国家:
美国
项目状态:
已结题
起止时间:
1984-04-01 至 1995-03-31
关键词:
adenosine triphosphate adenylate cyclase affinity chromatography antibody beta adrenergic receptor brain calcium calmodulin complementary DNA cyclic AMP enzyme inhibitors enzyme mechanism enzyme reconstitution enzyme structure fluorescence spectrometry genetic manipulation isoproterenol laboratory mouse laboratory rabbit membrane proteins molecular cloning protein kinase protein kinase A protein kinase C
中文摘要
细胞内cAMP控制着碳水化合物、脂质、蛋白质等方面
英文摘要
Intracelluar cAMP controls aspects of carbolydrate, lipid, protein
and nucleic acid metabolism in animal cells. Futhermore, cAMP
plays an important role for several types of neuronal function
including synaptic transmission, and the activity of specific ion
channels is regulated by cAMP. The levels of cAMP in brain are
directly controlled by adenylate cyclase which is regulated by
neurotransmitters and Ca2+. The long-term objectives of this
project are to elucidate the mechanisms for regulation of brain
adenylate cyclase by Ca2+, CaM, and cAMP dependent protein
kinase. Our laboratory has recently purified this enzyme to
homogeneity and obtained polyclonal antibodies against the CaM
sensitive adenylate cyclase front bovine brain. Furthermore, we
have recently discovered a protein from brain, designated pp90,
that activates brain adenylate cyclase which we suspect is a
calcium binding protein. Specific aims of this project include the
purification of CaM sensitive adenylate cyclase from bovine brain
using immunoaffinity chromatography, purification of the CaM
sensitive adenylate cyclase from brain, examination of the CaM,
Ca2+, and isoproterenol sensitivity of the CaM sensitive adenylate
cyclase reconstituted with beta adrenergic receptors, and
elucidation of the mechanism for regulation of the enzyme by
cAMP dependent protein kinase. Furthermore, we propose to
elucidate the mechanism for regulation of adenylate cyclase by
pp90. We will also attempt to clone cDNA's encoding the
sequence of the catalytic submit of the CaM sensitive adenylate
cyclase from bovine brain. This project should provide
fundemental information concerning the structure of adenylate
cyclases and the mechanisms for regulation of the enzyme by
Ca2+, protein kinases, and catecholamines.
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Role of PI3 Kinase and MAP Kinase in Memory Retrieval
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批准号:7620032
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项目类别:
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资助金额:$33.41万
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财政年份:2008
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负责人:DANIEL R STORM
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依托单位:
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批准号:8240050
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财政年份:2008
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Role of PI3 Kinase and MAP Kinase in Memory Retrieval
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批准号:8037101
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资助金额:$33.44万
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财政年份:2008
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负责人:DANIEL R STORM
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Role of PI3 Kinase and MAP Kinase in Memory Retrieval
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批准号:7522246
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资助金额:$33.67万
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批准号:7048100
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财政年份:2006
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批准号:8786106
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资助金额:$39.0万
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财政年份:2006
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批准号:7764779
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资助金额:$32.19万
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财政年份:2006
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资助金额:$30.71万
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批准号:8401162
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资助金额:$37.44万
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财政年份:2006
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依托单位:
Memory Enhancement by a Genetic Increase in cAMP Signals
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批准号:8600725
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项目类别:
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资助金额:$39.0万
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财政年份:2006
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负责人:DANIEL R STORM
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依托单位:
Memory Enhancement by A Genetic Increase in cAMP Signals
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批准号:7334744
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项目类别:
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资助金额:$32.19万
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财政年份:2006
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Memory Enhancement by a Genetic Increase in cAMP Signals
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批准号:8197421
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财政年份:2006
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依托单位:
Memory Enhancement by A Genetic Increase in cAMP Signals
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批准号:7172608
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项目类别:
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资助金额:$32.14万
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财政年份:2006
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依托单位:
Memory Enhancement by a Genetic Increase in cAMP Signals
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批准号:7980152
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项目类别:
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资助金额:$38.43万
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财政年份:2006
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依托单位:
Rgulation of vascular smooth muscle by CA2+ and CAMP
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批准号:6914215
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项目类别:
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资助金额:$28.69万
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财政年份:2004
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依托单位:
CALCIUM REGULATED ADENYLYL CYCLASE IN HEART
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批准号:6315349
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项目类别:
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资助金额:$19.12万
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财政年份:2000
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负责人:DANIEL R STORM
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依托单位:
REGULATION OF CAMP TRANSIENTS IN NEURONS
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批准号:6379494
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项目类别:
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资助金额:$23.11万
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财政年份:1999
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负责人:DANIEL R STORM
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依托单位:
REGULATION OF CAMP TRANSIENTS IN NEURONS
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批准号:6176109
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项目类别:
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资助金额:$22.45万
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财政年份:1999
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负责人:DANIEL R STORM
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依托单位:
Regulation of cAMP Transients in Neurons
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资助金额:$39.97万
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财政年份:1999
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依托单位:
海外基金