REGULATION OF PITUITARY OPIATE PEPTIDE SECRETION
REGULATION OF PITUITARY OPIATE PEPTIDE SECRETION
批准号:
2264124
负责人:
LINDA C SALAND
金额:
$9.82万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1986
资助国家:
美国
项目状态:
已结题
起止时间:
1986-08-01 至 1995-07-31
关键词:
bioassay catecholamines corticotropin releasing factor dopamine endorphins high performance liquid chromatography hormone regulation /control mechanism immunocytochemistry immunoperoxidase innervation laboratory rat ligands melanocyte stimulating hormone neural plasticity neuropeptide receptor neuropeptides neurotoxins neurotransmitter biosynthesis pituitary gland proopiomelanocortin radioimmunoassay secretion serotonin
中文摘要
主要目标是检验监管的可塑性程度
英文摘要
The major goals are to examine the extent of plasticity in regulatory
mechanisms for pituitary opiate peptide secretion, both in young rats, and
in aging animals. The major hypothesis to be tested is that innervation to
pro-opiomelanocortin (POMC) cells of the pituitary intermediate lobe
retains the ability to regenerate and regulate peptide secretion after
denervation in young individuals, and that during aging, there is
modulation of secretion as both nerves and endocrine cells undergo
potential degenerative alterations. Questions are: 1. How do POMC
endocrine cells respond to denervation and to potential re-innervation over
time? Young adult male rats will be treated with a catecholamine
neurotoxin, 6-hydroxydopamine (6-OHDA_, which induces neurite degeneration
in pituitary. Immunoreactive catecholamine and serotonin innervation will
be studied in drug treated and control animals over periods of 8 days, 3
and 6-8 weeks after treatment, to determine the extent of denervation, and
of potential re-innervation of endocrine cells. 2. Do POMC cells modify
their receptor population to adapt to denervation and potential re-
innervation? Pituitary tissue of 6-OHDA treated rats will be studied using
autoradiographic techniques with specific radioligands which bind to
dopamine-2 (D-2) and serotonin-2 (5-HT-2) receptors, for alterations in
receptor patterns which may occur with denervation and re-innervation. 3.
How do POMC cells respond to specific regulatory molecules after
denervation, and during potential re-innervation? Pituitaries of 6-OHDA
treated animals will be incubated in vitro to examine direct effects of
inhibitory or stimulatory molecules on peptides secretion. Peptides will
be measured by radioimmunoassay (RIA) and tissues studied by
immunocytochemical and electron microscopic (EM) techniques. 4. What
changes occur in POMC secretory cells and their innervation during normal
aging of individual animals? Pituitaries of aging rats (6, 12 and 18
months) will be examined for potential alterations in innervation patterns
as well as for modulation of peptide secretion, using immunocytochemistry
and RIA for tissue or blood samples. EM techniques will be used to
correlate cytology of aging pituitaries with alteration in immunoreactive
peptides. To determine if D-2 and 5-HT-2 receptors are altered with age,
autoradiographic patterns of ligand binding will be examined. 5. Do aging
POMC cells retain the ability to respond to regulatory molecules which
inhibit or stimulate secretion? Pituitaries from aging rats will be
studied in vitro using neurotransmitters and peptides which have been found
to modulate opiate peptide release in young animals. Opiate peptides have
significant effects on regulation of pain and on homeostatic mechanisms
related to stress. Investigations of plasticity of regulatory mechanisms,
in the forms of potential neurite regeneration and modulation of receptor
patterns on POMC cells, both in young and aging animals, will be a basis
for appropriate pharmacologic manipulation of opiate peptide secretion, in
animal models, and in man.
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Regulation of pituitary beta-endorphin secretion in aging rats: in vitro responsiveness to dopamine.
衰老大鼠垂体β-内啡肽分泌的调节:对多巴胺的体外反应。
DOI:
10.1016/0024-3205(95)00106-9
发表时间:
1995
期刊:
Life sciences
影响因子:
6.1
作者:
[Saland,LC, Samora,A, Apodaca,A, Ramirez,D]
通讯作者:
Ramirez,D
In vivo effects of serotonergic agents on alpha-melanocyte-stimulating hormone secretion.
血清素能药物对α-黑素细胞刺激激素分泌的体内影响。
DOI:
10.1159/000125968
发表时间:
1991
期刊:
Neuroendocrinology
影响因子:
4.1
作者:
[Carr,JA, Saland,LC, Samora,A, Benavidez,S, Krobert,K]
通讯作者:
Krobert,K
Organization of tyrosine hydroxylase-immunoreactive neurons in the di- and mesencephalon of the American bullfrog (Rana catesbeiana) during metamorphosis.
美国牛蛙(Rana catesbeiana)变态过程中二脑和中脑中酪氨酸羟化酶免疫反应性神经元的组织。
DOI:
10.1007/bf00318411
发表时间:
1991
期刊:
Cell and tissue research
影响因子:
3.6
作者:
[Carr,JA, Norris,DO, Samora,A]
通讯作者:
Samora,A
Immunocytochemical studies of tryptophan hydroxylase, tyrosine hydroxylase, and serotonin innervation in the aging rat neurointermediate pituitary.
衰老大鼠神经中间垂体色氨酸羟化酶、酪氨酸羟化酶和血清素神经支配的免疫细胞化学研究。
DOI:
10.1006/exnr.1993.1077
发表时间:
1993
期刊:
Experimental neurology
影响因子:
5.3
作者:
[Saland,LC, Samora,A, Sanchez,P, Chavez,G]
通讯作者:
Chavez,G
Effects of the enkephalin analog (D-Met2,Pro5)-enkephalinamide on alpha-melanocyte-stimulating hormone secretion.
脑啡肽类似物 (D-Met2,Pro5)-脑啡肽对 α-黑素细胞刺激激素分泌的影响。
DOI:
10.1016/0167-0115(93)90418-8
发表时间:
1993
期刊:
Regulatory peptides
影响因子:
--
作者:
[Carr,JA, Saland,LC, Samora,A, Tejeda,D]
通讯作者:
Tejeda,D
共 7 条
Chronic Ethanol Effects on CNS Opiate Receptors
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批准号:6506027
-
项目类别:
-
资助金额:$7.5万
-
财政年份:2002
-
负责人:LINDA C SALAND
-
依托单位:
Chronic Ethanol Effects on CNS Opiate Receptors
-
批准号:6630499
-
项目类别:
-
资助金额:$7.5万
-
财政年份:2002
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负责人:LINDA C SALAND
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依托单位:
REGULATION OF PITUITARY OPIATE PEPTIDE SECRETION
-
批准号:3402214
-
项目类别:
-
资助金额:$8.61万
-
财政年份:1986
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负责人:LINDA C SALAND
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依托单位:
REGULATION OF PITUITARY OPIATE PEPTIDE SECRETION
-
批准号:3402208
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项目类别:
-
资助金额:$7.97万
-
财政年份:1986
-
负责人:LINDA C SALAND
-
依托单位:
REGULATION OF PITUITARY OPIATE PEPTIDE SECRETION
-
批准号:3402211
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项目类别:
-
资助金额:$12.41万
-
财政年份:1986
-
负责人:LINDA C SALAND
-
依托单位:
REGULATION OF PITUITARY OPIATE PEPTIDE SECRETION
-
批准号:3402216
-
项目类别:
-
资助金额:$9.63万
-
财政年份:1986
-
负责人:LINDA C SALAND
-
依托单位:
REGULATION OF PITUITARY OPIATE PEPTIDE SECRETION
-
批准号:3402213
-
项目类别:
-
资助金额:$8.88万
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财政年份:1986
-
负责人:LINDA C SALAND
-
依托单位:
REGULATION OF PITUITARY OPIATE PEPTIDE SECRETION
-
批准号:3402215
-
项目类别:
-
资助金额:$13.37万
-
财政年份:1986
-
负责人:LINDA C SALAND
-
依托单位:
ULTRASTRUCTURE AND CONTROL OF ENDORPHIN CELLS
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批准号:3777929
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项目类别:
-
资助金额:$0.0万
-
财政年份:--
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负责人:LINDA C SALAND
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依托单位:
ULTRASTRUCTURE AND CONTROL OF ENDORPHIN CELLS
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批准号:3877456
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:LINDA C SALAND
-
依托单位:
ULTRASTRUCTURE AND CONTROL OF ENDORPHIN CELLS
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批准号:3841598
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:LINDA C SALAND
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依托单位:
ULTRASTRUCTURE AND CONTROL OF ENDORPHIN CELLS
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批准号:3755945
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:LINDA C SALAND
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依托单位:
ULTRASTRUCTURE AND CONTROL OF ENDORPHIN CELLS
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批准号:3856429
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:LINDA C SALAND
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依托单位:
海外基金