ANALYSIS OF FAST AXONAL TRANSPORT

快速轴突运输的分析

• 批准号: 3400107
• 负责人: ROGER D SLOBODA
• 金额: $ 14.11万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-09-01 至 1987-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3400107
• 关键词: Invertebrata; antibody; antimetabolites; birefringences; cytoskeleton; electrostimulus; fluorescence; fresh water environment; gel electrophoresis; microscopy; neuronal transport; neuropharmacology; neurotransmitters; saltwater environment; synaptic vesicles

The recent development of the AVEC methods of video-enhanced microscopy have made it possible to observe and record fast axonal transport of tubulovesicular elements in the axoplasm of large invertebrate neurons. In the giant axon of the squid, extruded axoplasm remains active for hours, making an ideal paradigm for many types of experiments. Thus it is now possible for the first time to design experiments aimed at a comprehensive analysis of the mechanisms of fast axonal transport. We have selected six invertebrate nerve preparations, each having some special features advantageous to this study. The AVEC methods will be used to characterize fast axonal transport in these six preparations and using them test a number of hypotheses regarding the mechanisms of fast axonal transport. Experimental approaches will include motion analysis, pharmacological experiments, the use of cytoskeletal proteins (or derivatives therof), antibodies to cytoskeletal proteins and mechanoenzymes, and metabolic inhibitors to influence fast axonal transport. Efforts will be made to disrupt and fractionate axoplasm under conditions sufficiently mild that motility can be retained by a single fraction or restored to a mixture of two or more fractions. The ultimate goal is to understand the process of fast axonal transport.

视频增强显微镜AVEC方法的最新进展 已经使得观察和记录快速轴突运输成为可能， 大型无脊椎动物神经元轴浆中的管状囊泡成分。 在 鱿鱼的巨大轴突，挤压的轴浆保持活跃数小时， 为许多类型的实验提供了理想的范例。 现在就是这样 第一次有可能设计实验，旨在全面 快速轴突运输的机制分析。 我们选出了六位 无脊椎动物的神经标本，每一种都有一些特殊的特征， 有利于这项研究。 AVEC方法将用于表征 在这六种制剂中快速轴突运输，并使用它们测试a 关于快速轴突运输机制的一些假设。 实验方法将包括运动分析，药理学 实验，细胞骨架蛋白（或其衍生物）的使用， 细胞骨架蛋白和机械酶的抗体，以及代谢 抑制剂影响快速轴突运输。 将努力 在足够温和的条件下破坏和破碎轴质， 运动性可由单一部分保留或恢复为以下的混合物： 两个或更多的分数。 最终目标是了解 快速轴突运输

项目成果

New observations on cell architecture and dynamics by video-enhanced contrast optical microscopy.

通过视频增强对比光学显微镜对细胞结构和动力学的新观察。

• DOI: 10.1146/annurev.bb.14.060185.001405
• 发表时间: 1985
• 期刊: Annual review of biophysics and biophysical chemistry
• 作者: Allen,RD

Detection of single microtubules in living cells: particle transport can occur in both directions along the same microtubule.

• DOI: 10.1083/jcb.99.5.1785
• 发表时间: 1984-11
• 期刊: The Journal of cell biology
• 作者: Hayden JH;Allen RD
• 通讯作者: Allen RD

Fast axonal transport by neurons from the jellyfish Cyanea capillata.

水母 Cyanea capillata 神经元的快速轴突运输。

• DOI: 10.1002/neu.480170104
• 发表时间: 1986
• 期刊: Journal of neurobiology
• 作者: Anderson,PA;Schwab,WE;Gilbert,S;Allen,RD
• 通讯作者: Allen,RD