NMR STUDIES OF HUMAN CEREBRAL METABOLISM IN VIVO

核磁共振波谱新技术 用~1H、~(13)C和~(31)P研究正常和无序 活体受试者的脑代谢。总而言之，这些 非侵入性方法是一种重复测量 在功能正常的神经组织中的特定化合物的方式可与 活组织检查，但没有组织损伤或死亡性改变。测量结果， 它将直接在大脑的不同部位产生 磷酸肌酸、三磷酸腺苷、 无机磷酸盐，细胞内pH，单酯和双酯的池 磷酸，高乳酸，N-乙酰天冬氨酸，化合物池 含有胆碱和肌酸，以及一些浓缩的氨基酸。这个 这项工作将在新设计的2.3特斯拉牛津/布鲁克光谱仪中完成 具有水质子成像能力；这台仪器将是第一个 它是一种。在对狗进行了一系列简短的实验后， 建立了光谱仪技术，以更大的机器，标准 将制定人类记录程序，并确定 正常光谱的特征和变化开始了。一项重大努力将 通过在大脑中定位光谱信号的来源 结合先进的光谱技术。痴呆症患者 多发性硬化症将被研究以获得关于 灰质和白质中神经元和神经胶质细胞的生物化学 这些细胞类型的比例因疾病而改变。病人 由于复杂的部分癫痫发作被考虑用于颞叶切除术， 中风患者或有中风风险的患者，以及脑瘤患者 研究评估核磁共振可测量量作为结果预测因子 以及管理层的助手。

Newly developed techniques in nuclear magnetic resonance spectroscopy (NMR) of 1H, 13C and 31p will be used to investigate normal and disordered cerebral metabolism in living human subjects. Collectively, these non-invasive methods are a means of making repeated measurements of specific compounds in functioning nervous tissue in a manner comparable to biopsy, but without tissue damage or agonal change. The measurements, which will be made directly in various parts of the brain are of the relative concentrations of phosphocreatine, adenosine triphosphate, inorganic phosphate, intracellular pH, pools of mono- and diesters of phosphoric acid, elevated lactate, N-acetylaspartate, pools of compounds containing choline and creatine, and some concentrated amino acids. The work will be done in a newly designed 2.3 Tesla Oxford/Bruker spectrometer with water proton imaging capability; this instrument will be the first of its kind. Following a short series of experiments on dogs to adapt established spectrometer techniques to the larger machine, standard procedures for human recording will be developed and determination of the characteristics and variation of normal spectra begun. A major effort will be made to localize the source of spectral signals within the brain by a combination of advanced spectroscopic techniques. Patients with dementia and multiple sclerosis will then be studied to gain new information about the biochemistry of neurons and glia in gray and white matter in which the proportions of these cell types have been altered by disease. Patients with complex partial seizures being considered for temporal lobectomy, patients with or at risk for stroke, and patients with brain tumors will be studied to evaluate NMR-measureeable quantities as predictors of outcome and adjuncts to management.