MICRO-ENVIRONMENTAL INFLUENCES ON PNS REGENERATION

这项研究的长期目标是在活体内测试 微环境对小鼠轴突再生的影响 周围神经系统。我们将继续发展解剖学和 将定量评估的生理学方法和技术 周围神经修复后的功能和解剖恢复。 具体目标I和II将量化感官的再生反应 运动神经元和它们的轴突。这一量化将进行 不同的实验条件系统地改变了 再生轴突的微环境。具体目标III-V重点放在 再生轴突与其靶点的相互作用。这些项目的目标是 实验是为了确定有多少轴突长回合适的位置 靶子，一旦进入靶子，有多少人建立了解剖和 有功能的生理连接。特定目标VI将使用 前面的量化方法的具体目的是直接比较 周围神经修复的各种标准程序的疗效。 这些研究的结果将提供量化的基线数据。 关于周围神经修复后的功能恢复。直接 功能神经的解剖测量与生理测量的相关性 再生将被用来比较不同外周的疗效 神经修复策略。这样的定量比较直接 人类周围神经修复的临床意义。

The long term objectives of this research are to test, in vivo, the influence of the microenvironment on axonal regeneration in the mouse peripheral nervous system. We will continue to develop anatomic and physiologic methods and techniques which will quantitatively assess functional as well as anatomical recovery following peripheral nerve repair. Specific Aims I and II will quantify the regeneration response of sensory and motor neurons and their axons. This quantification will be carried out for different experimental conditions which systematically alter the microenvironment of the regenerating axons. Specific Aims III-V focus on interactions of the regenerated axons with their target. The goal of these experiments is to establish how many axons grow back to their appropriate targets, and once in the target how many of them establish anatomical and physiological connections which are functional. Specific Aim VI will use the quantitative methods of the previous specific aims to directly compare the efficacy of various standard procedures for peripheral nerve repair. The results of these studies will provide quantitative baseline data concerning functional recovery following peripheral nerve repair. Direct correlation of anatomical and physiological measures of functional nerve regeneration will be used to compare the efficacy of different peripheral nerve repair strategies. Such quantitative comparisons have direct clinical relevance to human peripheral nerve repair.