HPLC ANALYSIS OF PURINE NUCLEOSIDES IN CREVICULAR FLUID

这项工作的长期目标是分析流体从 试图发现一种成分， 用于建立牙周组织健康状况的标准。 在此建议中，初步研究了高性能 描述了液相色谱（HPLC）技术对此问题的解决。 在 这些研究将收集龈沟液，并通过反向 用于嘌呤核碱基、核苷和 使用本实验室以前开发的技术。 根据初步研究，这些化合物应该存在并显示 口袋的深度和 磷酸化核苷。 该提案的具体目标包括：1） hplc技术的持续发展，包括对 固定相和移动的相，2）样品的分析 收集以评估技术的可重复性和3）分析样品 从唾液、血液和细菌中探索这个问题。 本研究的目的是将hplc技术应用于 牙周病提供临床有用的指南，以评估 牙周状况。

The long term objective of this work is to analyze the fluid from the gingival crevice of humans in an attempt to discover a component which can be used to establish criteria for the health status of the periodontium. In this proposal, initial studies on the application of high performance liquid chromatography (hplc) technology to this problem are described. In these studies crevicular fluid will be collected and analyzed by reverse phase and ion exchange hplc for purine nucleobases, nucleosides and nucleotides using techniques previously worked out in this laboratory. Based on preliminary studies, such compounds should present and should show that a correlation exists between pocket depth and the level of phosphorylated nucleosides. The specific aims of the proposal include 1) the continue development of the hplc technology including studies on both stationary and mobile phases, 2) the analysis of samples previously collected to assess reproductability of technique and 3) to analyze samples to explore the problem from saliva, blood and bacteria. It is the intent of this study to apply hplc technology to the problem of periodontal disease by providing clinically-useful guidelines to assess the status of the periodontium.