MULTIPLE ACTIVITY PATTERNS OF ACETYLCHOLINE RECEPTORS

这一建议所依据的主要意见是， 胚胎两栖类肌肉细胞，乙酰胆碱受体通道， 类似电导具有多种动力学形式。 具体目标是： 1)来量化控制发射器的分子速率常数 在不同动力学形式的通道中的结合和通道门控，2） 确定通道是否可以改变动力学模式，无论是作为个体还是作为 一个人口，和3）以确定是否和如何动力学heroeroevolutionin 胆碱能通道与肌细胞发育和胆碱能通道的组装有关。 神经肌肉突触 主要的实验方法是膜片钳技术 电生理学和单通道数据分析。 这项研究是 意义重大，因为它将补充结构-功能研究 在胆碱能通道的关系，它将提供深入了解 离子通道的动力学的分子和细胞机制 属性被调制，它将提供一个高分辨率的视图， 在肌肉运动过程中， 和突触发育。

The central observation upon which this proposal is based is that in embryonic amphibian muscle cells, acetylcholine receptor channels of similar conductance have multiple kinetic forms. The specific aims are: 1) to quantify the molecular rate constants which govern transmitter binding and channel gating in different kinetic forms of channel, 2) to determine if channels can change kinetic mode, either as individuals or as a population, and 3) to ascertain if and how kinetic hererogeneity in cholinergic channels relates to myocyte development and the assembly of the neuromuscular synapse. The major experimental method will be patch clamp electrophysiology and single-channel data analysis. The research is significant because it will compliment studies of structure-function relationships in cholinergic channels, it will give insight into the molecular and cellular mechanisms by which an ion channel's kinetic properties are modulated, and it will provide a high resolution view of changes in the properties of a transmitter-gated ion channel during muscle and synapse development.