GENERATION OF ANTISERUM TO PDGF SYNTHETIC PEPTIDES

血小板衍生生长因子(PDGF)是一种多肽激素，能够 创面愈合、动脉粥样硬化和 恶性的发展。受PDGF影响的细胞内事件包括 胶原和前列腺素的合成，癌基因的表达， 磷脂酰肌醇转换、酪氨酸磷酸化与细胞内 钙含量。目前的研究涉及促有丝分裂和趋化 骨肉瘤和血管细胞产生的PDGF样蛋白的作用。 为了继续这些研究，获得一份 识别特定生物活性区域的抗血清， 未减少的PDGF。由于大量提纯PDGF是不现实的 需要开发一种抗PDGF血清，提出了一种替代方法 这里。根据PDGF的氨基酸序列合成的多肽将被 合成的。这些多肽的构造将使次要的 结构由链内二硫键稳定。这种方法具有 成功地用来研制出一种与AFP交叉反应的抗血清 合成肽和一种天然的乙肝抗原。我们建议 合成四种不同的PDGF多肽，将其注射到兔体内 制备多克隆抗血清。然后将检测兔抗血清 用于识别合成肽和天然PDGF。

Platelet-derived growth factor, PDGF, is a polypeptide hormone capable of initiating processes involved in wound healing, atherosclerosis and the development of malignancy. Intracellular events influenced by PDGF include collagen and prostaglandin synthesis, oncogene expression, phosphatidylinositol turnover, tyrosine phosphorylation and intracellular calcium levels. Studies currently involve the mitogenic and chemotactic effects of PDGF-like proteins produced by osteosarcoma and vascular cells. In order to pursue these studies it would be extremely useful to obtain an antiserum which recognizes a defined region of biologically active, unreduced PDGF. Since it is not practical to purify PDGF in the quantity needed to develop an anti-PDGF serum, an alternative approach is presented here. Synthetic peptides based on the amino acid sequence of PDGF will be synthesized. These peptides will be constructed so that the secondary structure is stabilized by intrachain disulfide bridges. This approach has been successfully used to develop an antiserum which cross reacts with a synthetic peptide and a native Hepatitis B antigen. We propose to synthesize four distinct PDGF peptides, which will be injected into rabbits to generate a polyclonal antiserum. Rabbit antiserum will then be tested for recognition of the synthetic peptide and native PDGF.