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BIOPSY TECHNIQUE FOR STUDYING EXPERIMENTAL ENAMEL LESION

研究实验性牙釉质病变的活检技术

基本信息

批准号：
3425099
负责人：
THEODORE KOULOURIDES
金额：
$ 2.1万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
1986
资助国家：
美国
起止时间：
1986-03-01 至 1987-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3425099
关键词：
acid base balance biopsy calcium dental caries fluorine phosphorus tooth enamel

项目摘要

This application addresses the need to develop a technique for analysis of enamel lesions produced under various demineralizing and remineralizing conditions. These lesions exhibit zones of varying mineral density from the surface to the intact tissue. Our technique of abrasion biopsy will be adapted for the use of bovine enamel slabs following the principles used in an earlier investigation (Weatherell et al, 1985). The subsurface lesions will be produced on 3 x 5 mm enamel surfaces prepared with precise geometrical specifications. Two consecutive acid treatments will be applied. I. Immersion to acid buffers containing various amounts of fluoride (0.1 to 10 ppm). II. Immersion to fluoride-free buffers. Variables of acid concentration, pH, fluoride concentration, and exposure time will be studied for their influence on the development of lesions. The sampling will be conducted by abrasion of the lesion on Lapping Film in increments of 10 Mum measured with a sensitive micrometer. The abraded specimen will be dissolved in HC10 and analyzed for fluoride, phosphorus, and calcium content. On the basis of this data, each sampled layer will be defined in terms of mineral density, calcium to phosphorus ratio, and fluoride content. The abrasion biopsy technique will open possibilities for further studies of the demineralizing and remineralizing conditions "in vitro" and "in vivo" as they influence the development, progression, and possible arrestment of experimental lesions. Such knowledge may lead to a better understanding of the mechanisms of demineralization and remineralization, and possibly suggest better modalities for the clinical treatment of caries.

本申请解决了开发用于分析的技术的需要，在各种脱矿和矿化下产生的釉质损伤条件这些病变表现出不同的矿物质密度区，完整组织的表面。我们的磨损活检技术适用于牛牙釉质板的使用，早期的研究（Weatherell et al，1985）。皮下病变将在3 x 5 mm的搪瓷表面上生产，几何规格连续两次酸处理将应用。 I.浸入含有不同量的氟化物（0.1至10 ppm）。二.浸入无氟缓冲液中。酸浓度、pH值、氟化物浓度和暴露的变量将研究时间对病变发展的影响。将通过研磨胶片上的病变进行采样，用灵敏的千分尺测量10 μ m的增量。磨损将样品溶解在HCl中，并分析氟化物，磷，和钙含量。在此数据的基础上，每个采样层将根据矿物质密度、钙磷比定义，氟化物含量磨损活检技术将为进一步研究脱矿和矿化条件“，体外”和“体内”，因为它们影响发育、进展和可能是实验性损伤。这样的知识可能会导致更好地了解脱矿作用的机制，可能会建议更好的临床模式，治疗龋齿。