TRIGEMINAL NEURONS--SUSTAINED RESPONSE TO OPIOIDS

这个项目的长期目标是确定 神经功能障碍会导致慢性神经病理性疼痛。的目标是 这一建议是：(1)了解阿片类药物对心脏的调节作用 三叉神经细胞兴奋性氨基酸诱发(EAA)反应 (2)界定阿片类药物在产生和维持 对地产代理监管局的持续回应。我们假设阿片类药物的结合 激活G蛋白，进而提高第二信使的水平 在细胞内，并导致EAA诱发的反应改变。这个 实验旨在验证这一假设。 使用膜片钳测量EAA激活的电流 技术。实验将在三叉神经细胞上进行，标记为 从三叉神经脊束亚核分离的三叉神经丘脑细胞 尾侧和髓薄片中相同类型的神经元。 从这个项目中获得的信息将增进我们的理解 并帮助我们设计出更好的治疗方法 神经病理性疼痛。

The long term goal of this project is to determine the mechanism by which nerve dysfunction results in chronic neuropathic pain. The objective of this proposal is: (1) to understand the modulatory actions of opioids on excitatory amino acid-evoked (EAA) responses in trigeminal neurons; and (2) to define the role of opioids in generating and maintaining the sustained responses to EAA. We hypothesize that binding of an opioid activates G proteins which in turn raise the level of second messengers inside the cell and result in a change in the EAA-evoked responses. The experiments are designed to test this hypothesis. The EAA-activated currents will be measured using the patch clamp technique. Experiments will be performed on trigeminal neurons, labeled trigeminothalamic cells isolated from the spinal trigeminal subnucleus caudalis and the same type of neurons in thin medullary slices. The information obtained from this project will advance our understanding of the pain mechanism and help us design a better therapy for treatment of neuropathic pain.