DEVELOPMENT OF DUCTAL CELL CULTURE FROM PAROTID GLAND

腮腺导管细胞培养物的发育

• 批准号: 3425863
• 负责人: EILEEN L WATSON
• 金额: $ 3.7万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1994-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3425863
• 关键词: basement membrane; beta adrenergic agent; biological models; biomarker; calcium flux; cell cycle; cell type; centrifugation; chloride channels; cholinergic agents; cyclic AMP; electron microscopy; guanine nucleotide binding protein; kallikreins; keratin; laboratory rat; light microscopy; model design /development; parotid gland; physical separation; tissue /cell culture

The goal of the research proposed is to develop and evaluate nontransformed ductal cell cultures of rat parotid glands to be used as models for the in vitro study of the physiological and pathophysiological changes that occur in exocrine gland cells. The objective will be the long-term culture of ductal cells and the passaging of these cells, thereby making cells available for study over a longer period than an acute experiment. Because cells tend to dedifferentiate in culture, ductal cells will be isolated from dispersed cell preparations prior to culturing while the cells still have their characteristic morphology. Ductal cells will be isolated and analyzed biochemically to obtain baseline data. The rat parotid gland has been chosen for these studies because the majority of the ducts are striated (Redman, 1987; Robinovitch, 1967) and striated ducts are important in regulation of electrolytes (Schneyer et al., 1972). Isolated ductal cells will be grown in basement membrane coated culture dishes. The resulting cultures will be morphologically and biochemically characterized. To insure that the cells in culture are ductal in origin, antibodies to ductal cell markers will be used.

本研究的目的是开发和评价非转化的 大鼠腮腺导管细胞培养物可作为研究炎症的模型 体外研究发生的生理和病理生理变化 在外分泌腺细胞中。 目标将是长期培养 导管细胞和这些细胞的传代，从而使细胞 可用于比急性实验更长时间的研究。 因为 细胞倾向于在培养中去分化，导管细胞将被分离 从培养前的分散的细胞制备物， 有其特有的形态。 将分离导管细胞， 进行生化分析以获得基线数据。 大鼠腮腺有 之所以选择这些研究，是因为大多数管道都是 纹状管（Redman，1987; Robinovitch，1967）和纹状管是重要的 在电解质的调节中（Schneyer等人，1972年）。 孤立导管 细胞将在基底膜包被的培养皿中生长。 的 将对所得培养物进行形态学和生物化学表征。 为了确保培养物中的细胞来源于导管，需要使用抗 将使用导管细胞标记物。