STRUCTURE OF HEPATITIS B CORE AND E PROTEINS
STRUCTURE OF HEPATITIS B CORE AND E PROTEINS
批准号:
3432556
负责人:
Darrell L Peterson
金额:
$2.47万
依托单位国家:
美国
项目类别:
财政年份:
1992
资助国家:
美国
项目状态:
已结题
起止时间:
1992-09-10 至 1995-09-09
中文摘要
本项目的总体目标是阐明
B型肝炎核心抗原(HBcAg)与
B e型肝炎(HBeAg)抗原。 这些抗原是一种复合物的一部分
B型肝炎病毒抗原和宿主抗体系统,
描述人类感染这种病毒的特征。 存在这些
抗原和抗体广泛用于诊断感染,
也用于疾病的预后。 HBeAg蛋白,分享其大部分
氨基酸序列与HBcAg相同,但抗原性不同。
这种抗原性差异必然反映了蛋白质结构的差异。
虽然HBeAg在疾病中的作用尚不清楚,但它已被广泛应用。
最近表明,它可能在慢性疾病的发展中发挥作用。
新生儿感染期间感染。 更详细地理解
这种蛋白质的结构可以更好地了解其
功能
这两种蛋白质之间的结构和抗原关系将
使用酿酒酵母表达系统进行研究。 两
将使用不同的表达载体。 一个指导合成,
蛋白质的细胞内积累,而另一个进行
蛋白质的合成和分泌。 蛋白质对应于
HBCAG、HBeAg和这些的突变体将从每一种中产生和纯化。
这些系统,并受到物理,化学和免疫化学
分析. 这些研究将使我们能够确定
环境(细胞内与细胞外)的物理和
这些蛋白质的抗原结构。
英文摘要
The overall goal of this project is to elucidate the structural basis for
the antigenic differences between the hepatitis B core (HBcAg) and
Hepatitis B e (HBeAg) antigens. These antigens are part of a complex
system of Hepatitis B viral antigens and host antibodies which
characterize human infection by this virus. The presence of these
antigens and anti-bodies are widely used for diagnosis of infection and
also for prognosis of the disease. HBeAg protein, shares most of its
amino acid sequence with the HBcAg, and yet is antigenically distinct.
Such antigenic differences must reflect protein structural differences.
Although the function of HBeAg during the disease is unknown, it has been
recently suggested that it may play a role in the development of chronic
infection during neonatal infection. A more detailed understanding of
the structure of this protein may provide better insight into its
function.
The structural and antigenic relationship between these two proteins will
be investigated using Saccharomyces cerevisiae expression systems. Two
different expression vectors will be used. One directs synthesis and
intra-cellular accumulation of the protein while the other carries out
synthesis and secretion of the protein. Proteins corresponding to the
HBCAG, HBeAg and mutants of these will be produced and purified from each
of these systems and subjected to physical, chemical, and immunochemical
analysis. These studies will allow us to determine the role of the
environment (intracellular vs extracellular) on the physical and
antigenic structure of these proteins.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
A RECOMBINANT HEPETITIS C VACCINE
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批准号:6055125
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项目类别:
-
资助金额:$10.0万
-
财政年份:2000
-
负责人:Darrell L Peterson
-
依托单位:
STRUCTURE OF HEPATITIS B CORE AND E PROTEINS
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批准号:2291604
-
项目类别:
-
资助金额:$2.47万
-
财政年份:1992
-
负责人:Darrell L Peterson
-
依托单位:
STRUCTURE OF HEPATITIS B CORE AND E PROTEINS
-
批准号:3432557
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项目类别:
-
资助金额:$2.47万
-
财政年份:1992
-
负责人:Darrell L Peterson
-
依托单位:
THE STRUCTURE OF HEPATITIS B ANTIGENS
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批准号:3126512
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项目类别:
-
资助金额:$12.15万
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财政年份:1991
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负责人:Darrell L Peterson
-
依托单位:
THE STRUCTURE OF HEPATITIS B ANTIGENS
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批准号:3126508
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项目类别:
-
资助金额:$11.52万
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财政年份:1991
-
负责人:Darrell L Peterson
-
依托单位:
STRUCTURE OF HEPATITIS B ANTIGENS
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批准号:2060318
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项目类别:
-
资助金额:$12.39万
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财政年份:1991
-
负责人:Darrell L Peterson
-
依托单位:
STRUCTURE OF HEPATITIS B ANTIGENS
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批准号:3126511
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项目类别:
-
资助金额:$11.87万
-
财政年份:1991
-
负责人:Darrell L Peterson
-
依托单位:
STRUCTURE OF HEPATITIS B ANTIGENS
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批准号:2060317
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项目类别:
-
资助金额:$11.76万
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财政年份:1991
-
负责人:Darrell L Peterson
-
依托单位:
STRUCTURE OF HEPATITIS B ANTIGENS
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批准号:3126509
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项目类别:
-
资助金额:$9.89万
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财政年份:1979
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负责人:Darrell L Peterson
-
依托单位:
STRUCTURE OF HEPATITIS B ANTIGENS
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批准号:3126510
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项目类别:
-
资助金额:$10.32万
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财政年份:1979
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负责人:Darrell L Peterson
-
依托单位:
STRUCTURE OF HEPATITIS B ANTIGENS
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批准号:3126505
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项目类别:
-
资助金额:$7.68万
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财政年份:1979
-
负责人:Darrell L Peterson
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依托单位:
国内基金
海外基金
Mettl3/Syk/MAPK通路调控中性粒细胞胞
外诱捕网 (neutrophil extracellular
traps, NETs)的形成对脓毒症急性肺损
伤影响的分子机制研究
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批准号:
-
项目类别:省市级项目
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资助金额:10.0万元
-
批准年份:2025
-
负责人:罗舒华
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依托单位: