TOPOGRAPHY OF GLYCOSYLATION IN THE ENDOPLASMIC RETICULUM

申请资助的目的是让 糖基化反应的拓扑学合作研究 卡洛斯赫希伯格博士的实验室 （马萨诸塞州伍斯特市马萨诸塞州大学医学中心）和Armando博士 Parodi实验室（丰达西翁Compomar基金会，阿根廷布宜诺斯艾利斯）。 的 该提案的长期目标是了解前体如何在 蛋白质和脂质糖基化可以跨膜转运， 内质网，从胞质侧到内腔侧。 这种易位被认为在以下方面发挥重要的调节作用： 蛋白质和脂质糖基化。 该提案的具体目的是确定是否多利科尔-P- 葡萄糖在内质网的胞质侧合成 膜，如果是这样，通过什么机制，这种脂糖随后 转移穿过膜进入管腔，在那里它充当 用于进一步糖基化反应的底物。 这些研究将 补充赫什伯格博士实验室正在进行的研究， 细胞生物学，生物化学和糖基化反应的分子生物学， 与帕罗迪博士的实验室合作研究长萜醇的化学和生物化学 低聚糖。

The purpose of this application for grant support is to allow a collaborative study on the topography of glycosylation reactions in the rough endoplasmic reticulum between Dr. Carlos Hirschberg's laboratory (University of Massachusetts Medical Center, Worcester, MA) and Dr. Armando Parodi's laboratory (Fundacion Compomar, Buenos Aires, Argentina). The long term goals of this proposal are to understand how precursors in protein and lipid glycosylation can be translocated across the membrane of the endoplasmic reticulum, from the cytosolic side to the lumenal side. This translocation is believed to play an important regulatory role in protein and lipid glycosylation. The Specific Aims of this proposal are to determine whether dolichol-P- glucose is synthesized on the cytosolic side of the endoplasmic reticulum membrane and if so, by what mechanism this lipid-saccharide is subsequently translocated across the membrane into the lumen where it serves as a substrate for further glycosylation reactions. These studies will complement ongoing research in Dr. Hirschberg's laboratory dealing with the cell biology, biochemistry & molecular biology of glycosylation reactions, with Dr. Parodi's laboratory on the chemistry and biochemistry of dolichol oligosaccharides.