TOPOGRAPHY OF GLYCOSYLATION IN THE ENDOPLASMIC RETICULUM

这项赠款支持申请的目的是允许 糖基化反应拓扑学的协同研究 卡洛斯·赫希伯格博士实验室之间的粗面内质网 (马萨诸塞州伍斯特马萨诸塞大学医学中心)和阿曼多博士 Parodi的实验室(Fundacion Compomar，阿根廷布宜诺斯艾利斯)。这个 这项提案的长期目标是了解前兆如何在 蛋白质和脂肪糖基化可以跨膜转运。 内质网，从胞浆侧到管腔侧。 这种易位被认为在人类免疫缺陷中起着重要的调节作用 蛋白质和脂肪糖基化。 这项提案的具体目的是确定多效酚-P- 葡萄糖是在内质网的胞浆侧合成的 膜，如果是这样，这种脂糖随后是通过什么机制 跨膜转移到管腔中，在那里它充当 进一步糖基化反应的底物。这些研究将 补充赫希伯格博士实验室正在进行的研究 糖基化反应的细胞生物学、生物化学和分子生物学， 与帕罗迪博士的实验室一起研究二羟苯二酚的化学和生物化学 低聚糖。

The purpose of this application for grant support is to allow a collaborative study on the topography of glycosylation reactions in the rough endoplasmic reticulum between Dr. Carlos Hirschberg's laboratory (University of Massachusetts Medical Center, Worcester, MA) and Dr. Armando Parodi's laboratory (Fundacion Compomar, Buenos Aires, Argentina). The long term goals of this proposal are to understand how precursors in protein and lipid glycosylation can be translocated across the membrane of the endoplasmic reticulum, from the cytosolic side to the lumenal side. This translocation is believed to play an important regulatory role in protein and lipid glycosylation. The Specific Aims of this proposal are to determine whether dolichol-P- glucose is synthesized on the cytosolic side of the endoplasmic reticulum membrane and if so, by what mechanism this lipid-saccharide is subsequently translocated across the membrane into the lumen where it serves as a substrate for further glycosylation reactions. These studies will complement ongoing research in Dr. Hirschberg's laboratory dealing with the cell biology, biochemistry & molecular biology of glycosylation reactions, with Dr. Parodi's laboratory on the chemistry and biochemistry of dolichol oligosaccharides.