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TGFB EXPRESSION DURING EMBRYO RECEPTIVITY AND CELL DEATH

TGFB EXPRESSION DURING EMBRYO RECEPTIVITY AND CELL DEATH
胚胎接受和细胞死亡过程中 TGFB 的表达
批准号:
3426731
负责人:
JOY I MULHOLLAND
金额:
$5.0万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1991
资助国家:
美国
项目状态:
已结题
起止时间:
1991-05-01 至 1993-04-30

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中文摘要
翻译
尽管哺乳动物子宫中的许多事件都依赖于类固醇激素,
英文摘要
Although many events in the mammalian uterus are steroid hormone dependent, little is known about the cellular mechanisms through which estradiol and progesterone direct specific uterine functions. The proposed research will determine if certain actions of steroid hormones on different uterine cell types could be mediated by transforming growth factor beta (TGFB). Since it is not yet known if TGFB is regulated by steroid hormones in the uterus, synthesis of this protein will be examined during two important uterine functions which are directed by distinct hormonal milieux; receptivity to embryo attachment and cell death. TGFB transcription and synthesis will be examined in separated luminal epithelial and stromal cells from the rat uterus under hormonal conditions which give rise to each of these functions. Uterine receptivity will be induced in ovariectomized rats by a series of progesterone and estradiol injections which mimic the hormonal conditions of early pregnancy. Cell death will be induced by ovariectomy. TGFB transcription will be measured using northern and RNA slot-blot hybridization analyses of cytoplasmic RNA as well as transcription and nuclear run-off assays of newly transcribed RNA in cell nuclei. Synthesis of TGFB will be analyzed by gel-electrophoresis and immunoblotting of uterine cell proteins. The results of these experiments should demonstrate whether a change in TGFB synthesis is associated either with receptivity to embryo attachment or with cell death, if TGFB synthesis is regulated by steroid hormones in specific uterine cell types, and whether this regulation is transcriptional, posttranscriptional, or translational. If a role for TGFB in establishing receptivity for embryo implantation in the rat is indicated by this research, a firm precedent would be established for future investigations to determine if TGFB serves the same function in other mammals and if it is involved in human infertility.
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FUNCTION OF HB/EGF IN UTERINE STROMAL CELLS
  • 批准号:
    6181762
  • 项目类别:
  • 资助金额:
    $25.23万
  • 财政年份:
    1998
  • 负责人:
    JOY I MULHOLLAND
  • 依托单位:
FUNCTION OF HB/EGF IN UTERINE STROMAL CELLS
  • 批准号:
    2669662
  • 项目类别:
  • 资助金额:
    $23.84万
  • 财政年份:
    1998
  • 负责人:
    JOY I MULHOLLAND
  • 依托单位:
FUNCTION OF HB/EGF IN UTERINE STROMAL CELLS
  • 批准号:
    2889538
  • 项目类别:
  • 资助金额:
    $24.5万
  • 财政年份:
    1998
  • 负责人:
    JOY I MULHOLLAND
  • 依托单位:
HB EGF AS A BIOMARKER OF THE ENDOTHELIUM-DEPENDENT NO PA
  • 批准号:
    2658169
  • 项目类别:
  • 资助金额:
    $7.96万
  • 财政年份:
    1997
  • 负责人:
    JOY I MULHOLLAND
  • 依托单位:
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