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SEQUENCE AND REGULATION OF VITAMIN K BIOSYNTHESIS GENES

维生素 K 生物合成基因的序列和调控

基本信息

批准号：
3438827
负责人：
RANGASWAMY MEGANATHAN
金额：
$ 9.91万
依托单位：
NORTHERN ILLINOIS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1989
资助国家：
美国
起止时间：
1989-04-01 至 1993-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3438827
关键词：
Escherichia coli gene deletion mutation gene expression linkage mapping menadione mutant nucleic acid sequence operon vitamin biosynthesis

项目摘要

The principal aim of this investigation is to gain insight into the regulation of bacterial vitamin K biosynthesis by the molecular examination and dissection of the structure and organization of vitamin K biosynthetic genes. Vitamin K is an important fat soluble vitamin which plays a significant role in human and animal health. Its deficiency leads to several significant clinical pathologies such as hypoprothrombinemia. In healthy individuals the bulk of the vitamin K requirement is met by bacterial biosynthesis of intestinal flora. The pathway for the vitamin k napthoquinone nucleus is reasonably understood, and the genes involved in the synthesis of the various enzymes have been mapped and cloned. The synthesis of the vitamin is dependent upon the aromatic amino acid pathway and utilizes isochorismate and 2-ketoglutarate as initial reactants. The identified intermediates are 2-succinyl-6-hydroxy-2,4- cyclohexadiene-1-carboxylate (SHCHC), o-succinylbenzoate (OSB) ester of OSB (OSB-CoA), 1,4-dihydroxy-2-naphthoate (DHNA) and desmethylmenaquinone (DMK). The menA gene is located at 88 min and the menBCDE gene cluster at 48.5 min of E. coli linkage map. The men cluster has been cloned into pBR322 and the DNA sequence of menD determined. This investigation will determine the sequence of the remaining men cluster genes and initiate studies of the regulation of these genes using lacZ fusions.

本次调查的主要目的是深入了解分子调节细菌维生素K生物合成检查和剖析结构和组织维生素 K 生物合成基因。维生素K是一种重要的脂肪可溶性维生素，对人类和动物起着重要作用健康。它的缺陷导致了一些重大的临床问题病理如低凝血酶原血症。在健康个体中大部分维生素 K 需求由细菌满足肠道菌群的生物合成。维生素 K 萘醌核的途径是合理的理解，以及参与各种合成的基因酶已被绘制和克隆。维生素的合成取决于芳香族氨基酸途径并利用异分支酸和2-酮戊二酸作为初始反应物。这确定的中间体是2-琥珀酰-6-羟基-2,4- 1-环己二烯-1-羧酸酯 (SCHHC)、邻琥珀酰苯甲酸酯 (OSB) OSB (OSB-CoA)、1,4-二羟基-2-萘甲酸酯 (DHNA) 和去甲基甲萘醌（DMK）。 menA 基因位于 88 分钟，menBCDE 基因簇位于 48.5 分钟的大肠杆菌连锁图。男性集群已被克隆进入pBR322并确定menD的DNA序列。这调查将确定剩余人员的顺序聚类基因并启动这些基因调控的研究使用 lacZ 融合。