TRANSPORT & METABOLISM OF D-AMINO ACIDS BY MAMMALS

运输

• 批准号: 3437832
• 负责人: ROBERT L PRESTON
• 金额: $ 6.93万
• 依托单位: ILLINOIS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-30 至 1990-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3437832
• 关键词: aminoacid; aminoacid metabolism; aminoacid transport; brush border membrane; high performance liquid chromatography; intestines; isomer; kidney metabolism; nutrition related tag; renal toxin

It has been recently demonstrated in my laboratory that D-amino acids occur fairly commonly in marine invertebrates. Some of these invertebrate species are used as food by humans and other mammals. It is usually assumed that D-amino acids are inactive biologically. However, in certain marine invertebrate species D- amino acids are accumulated by specific transport systems and metabolized. This suggests that D-amino acids may have some normal physiological role in these species. The function of D- amino is currently under investigation but it appears D-amino acids function in osmoregulation and as nutritional resources. D-amino acids may occur in seafood and also in highly processed food which causes racemization of L-amino acids. They are also produced and released by bacterial flora which normally reside in the intestine. It is quite possible that the tissues of mammals also contain D-amino acids and are capable of transporting and metabolizing them. The objective of this project is to test the hypothesis that D-amino acids are physiologically significant in mammals. This will be done by the following methods: surveying a variety of mammalian species for the presence of D-amino acids in their tissues, measuring the transport properties of rabbit and rat intestine and kidney for D-amino acids, and measuring the capacity of mammalian tissues to metabolize D-amino acids. Preliminary experiments will also be conducted on the toxicity of D-amino acids. To my knowledge this is the first comprehensive attempt to examine the role of D-amino acids in mammalian physiology. The findings of this study will be used to support a future proposal for an in depth study of the role of D-amino acids in mammalian tissues.

最近在我的实验室里证明， 酸在海洋无脊椎动物中相当常见。 一些 这些无脊椎动物被人类和其他动物用作食物。 哺乳动物 通常认为D-氨基酸是无活性的 从生理上来说 然而，在某些海洋无脊椎动物物种中，D- 氨基酸通过特定的运输系统积累， 新陈代谢。 这表明D-氨基酸可能具有一些 正常的生理作用。 D的功能- 氨基目前正在调查中，但似乎D-氨基 酸在调节血糖中起作用并作为营养资源。 D-氨基酸可能存在于海鲜和高度加工的食品中， 导致L-氨基酸外消旋化的食物。 他们也是 由细菌植物群产生和释放， 肠子 很有可能哺乳动物的组织也 含有D-氨基酸，能够运输， 代谢它们。 本项目的目标是测试 假设D-氨基酸在生理上重要， 哺乳动物 这将通过以下方法完成： 多种哺乳动物物种中存在D-氨基酸 在它们的组织中，测量兔和 大鼠肠和肾脏的D-氨基酸，并测量 哺乳动物组织代谢D-氨基酸的能力。 还将进行初步的毒性实验， D-氨基酸 据我所知，这是第一次全面尝试， 研究D-氨基酸在哺乳动物生理学中的作用。 的 这项研究的结果将用于支持未来的建议， D-氨基酸在哺乳动物中作用的深入研究 组织中