NONHUMAN PRIMATE: DEVELOPMENT OF LIPOPROTEIN SUBCLASSES

这是一个试点项目，旨在调查 食蟹猴NHLBI的脂蛋白亚类 支持鲍曼·格雷医学院的灵长类中心。 这项研究的长期目标是了解 成体亚类的异质性已经进化，无论亚类 模式是在早期生命中可检测到的可遗传特征，以及 幼年的亚类模式是早期的标志 检测到心血管疾病风险增加。特定的 这项研究中要解决的问题旨在获得 关于高密度脂蛋白和低密度脂蛋白亚类分布的初步数据 发育的功能和包括：(1)脂蛋白是如何 新生儿和成人的亚类分布不同； (2)亚类分布在出生后多长时间内假定为成年 特征；(3)两者之间有什么关系 载脂蛋白和血脂水平及特异性高密度脂蛋白和低密度脂蛋白 亚类；以及(4)可以检测到 与高胆固醇血症和低胆固醇血症有关 挑战饮食胆固醇的动物？高密度脂蛋白和低密度脂蛋白 亚类的异质性将在来自特定的子代上进行评估 低胆固醇血症的猴子之间的交配 饮食中胆固醇的反应者和那些 高胆固醇血症对饮食胆固醇的反应。子类 分布将通过非变性梯度凝胶进行评估 在早期发育的几个时间点上的电泳法， 包括：出生；产后3、7、14和28天；以及3、6和12天 月份。模式将与脂质和载脂蛋白相关 水平和载脂蛋白AI、B、E的分布为 经Western blotting鉴定。这项研究应该提供 关于是否有可能在生命早期识别出 脂蛋白亚类可能被认为是高血压的危险因素 心血管疾病的发展。

This is a pilot project to investigate the development of lipoprotein subclasses in cynomolgus monkeys at the NHLBI- supported primate center at Bowman Gray School of Medicine. The long-range objectives of the study are to understand how adult subclass heterogeneity has evolved, whether subclass patterns are heritable traits detectable in early life, and whether subclass patterns in the young serve as markers for the early detection of increased risk to cardiovascular disease. Specific questions to be addressed in this study are designed to obtain preliminary data on HDL and LDL subclass distribution as a function of development and include: (1) how does lipoprotein subclass distribution in the neonate differ from that of the adult; (2) how soon after birth does the subclass profile assume adult characteristics; (3) what is the relationship between apolipoprotein and lipid levels and specific HDL and LDL subclasses; and (4) can one detect HDL and LDL subclasses which are related to the hyper- and hypocholesterolemic response in animals challenged with dietary cholesterol? HDL and LDL subclass heterogeneity will be evaluated on progeny from specific matings between monkeys which are hypocholesterolemic responders to dietary cholesterol and those which are hypercholesterolemic responders to dietary cholesterol. Subclass distribution will be evaluated by non-denaturing gradient gel electrophoresis at several time points during early development, including: birth; 3, 7, 14, and 28 days postpartum; and 3, 6, and 12 months. Patterns will be correlated with lipid and apolipoprotein levels and with apolipoprotein AI, B, and E distribution as determined by Western blotting. The study should provide preliminary data on whether it is possible to identify, early in life, lipoprotein subclasses which might be deemed risk factors in the development of cardiovascular disease.