ORGANIZATION AND EXPRESSION OF B-TUBULIN GENES

这些研究将检查基因的组织和表达 在水生真菌Allomyces macrogynus中的b-微管蛋白。 微管蛋白 构成减数分裂和有丝分裂器的主要部分， 鞭毛，并参与细胞内运输，在细胞膜 形成和确定细胞形状。 我们将确定 这种蛋白质的不同形式是否具有不同的功能， 在生命周期的不同时期表现出来。 本组织和 基因的结构将通过Southern印迹、克隆 微管蛋白基因在噬菌体或质粒载体中的用途， 双脱氧核苷酸测序技术。 功能与发展 微管蛋白的调节将通过分离微管蛋白突变体来研究， 进行连锁互补分析。 微管蛋白将是 在2D凝胶上表征。 基因表达的发育分析将 也可以通过北方印迹杂交研究进行。 阐明 这些基因的遗传结构和表达调控将 增加我们对细胞骨架在 控制细胞的分化，以及如何在所有的发展过程中， 有机体受到控制。

These studies will examine the organization and expression of the gene(s) for b-tubulin in the aquatic fungus, Allomyces macrogynus. Tubulin composes the major portion of the meiotic and mitotic apparatus and the flagella and is involved in intra-cellular transport, in cell membrane formation and in the determination of cell shape. We will determine whether different forms of this protein have different functions or are expressed during different times of the life cycle. The organization and structure of the gene(s) will be determined by Southern blotting, cloning of the tubulin genes in either a phage or plasmid vector and use of the dideoxy nucleotide sequencing technique. The function and developmental regulation of the tubulins will be studied by isolating tubulin mutants and performing linkage and complementation analysis of them. Tubulins will be characterized on 2D gels. Developmental analysis of gene expression will also be performed by Northern blot hybridization studies. Elucidation of the genetic structure and regulation of expression of these genes will increase our understanding of the role of the cell cytoskeleton in controlling cellular differention and of how developmental processes in all organisms are controlled.