SYNTHESIS OF N-ACETYLGALACTOSAMINE IN ENCYSTING GIARDIA
SYNTHESIS OF N-ACETYLGALACTOSAMINE IN ENCYSTING GIARDIA
批准号:
3436813
负责人:
EDWARD L JARROLL
金额:
$9.06万
依托单位国家:
美国
项目类别:
财政年份:
1991
资助国家:
美国
项目状态:
已结题
起止时间:
1991-06-01 至 1994-05-31
关键词:
Giardia N acetylgalactosamine carbohydrate biosynthesis communicable disease diagnosis communicable disease transmission diagnosis design /evaluation diagnosis quality /standard drug design /synthesis /production enzyme mechanism gas chromatography giardiasis mass spectrometry microorganism culture microorganism growth molecular pathology nuclear magnetic resonance spectroscopy parasitic gastrointestinal disorder protozoal cyst radiotracer
中文摘要
描述(改编自申请人摘要):贾第鞭毛虫是主要的
英文摘要
DESCRIPTION (Adapted from the Applicat's Abstract): Giardia is the major
cause of infectious waterborne intestinal disease in humans. Nevertheless,
little is known about the basic biology of Giardia, specifically, its
mechanism of encystment, which is required for parasite transmission from
one host to the next. Thus, the overall objective of this project is to
provide detailed knowledge on pathway(s) of synthesis of the cyst
wall-specific amino hexose, N-acetylgalactosamine (GalNAc), required for
and specifically induced during Giardia encystment. This overall objective
will be accomplished by completing the following specific aims: 1)
Determination of the possible pathways of GalNAc synthesis using isotopic
labeling of in vitro encysting Giardia trophozoites with known GalNAc
precursors. 2) Characterization of key GalNAc synthetic enzymes with
respect to their kinetics, substrate specificity, and sensitivity to known
inhibitors.
Completion of this project should give important information for pursuing
the following long range goals: 1) to understand the mechanism of
encystment and its regulation at the molecular level, 2) to develop new
chemotherapy targeting GalNAc synthesis or other encystment pathways, and
3) to improve diagnostic methods for detecting Giardia in human and
environmental samples.
期刊论文(3)
专著(0)
科研奖励(0)
会议论文
Galactosamine-synthesizing enzymes are induced when Giardia encyst.
当贾第鞭毛虫包囊时,会诱导半乳糖胺合成酶。
DOI:
10.1016/0166-6851(92)90179-n
发表时间:
1992
期刊:
Molecular and biochemical parasitology
影响因子:
1.5
作者:
[Macechko,PT, Steimle,PA, Lindmark,DG, Erlandsen,SL, Jarroll,EL]
通讯作者:
Jarroll,EL
NOVEL NEW ENZYME & PRODUCT FROM ENCYSTING GIARDIA
-
批准号:6345224
-
项目类别:
-
资助金额:$0.75万
-
财政年份:2000
-
负责人:EDWARD L JARROLL
-
依托单位:
GIARDIA ENCYSTMENT: CONTROL OF GA1NAC SYNTHESIS
-
批准号:6157624
-
项目类别:
-
资助金额:$15.29万
-
财政年份:2000
-
负责人:EDWARD L JARROLL
-
依托单位:
NOVEL NEW ENZYME & PRODUCT FROM ENCYSTING GIARDIA
-
批准号:6478948
-
项目类别:
-
资助金额:$5.36万
-
财政年份:2000
-
负责人:EDWARD L JARROLL
-
依托单位:
NOVEL NEW ENZYME & PRODUCT FROM ENCYSTING GIARDIA
-
批准号:6206419
-
项目类别:
-
资助金额:$0.75万
-
财政年份:1999
-
负责人:EDWARD L JARROLL
-
依托单位:
NOVEL NEW ENZYME & PRODUCT FROM ENCYSTING GIARDIA
-
批准号:6123261
-
项目类别:
-
资助金额:$0.0万
-
财政年份:1998
-
负责人:EDWARD L JARROLL
-
依托单位:
NOVEL NEW ENZYME & PRODUCT FROM ENCYSTING GIARDIA
-
批准号:6254146
-
项目类别:
-
资助金额:$1.96万
-
财政年份:1997
-
负责人:EDWARD L JARROLL
-
依托单位:
NOVEL NEW ENZYME AND PRODUCT FROM ENCYSTING GIARDIA
-
批准号:2077285
-
项目类别:
-
资助金额:$11.58万
-
财政年份:1996
-
负责人:EDWARD L JARROLL
-
依托单位:
海外基金