COLUMN PACKINGS FOR FAST HPLC OF BIOMOLECULES

用于生物分子快速 HPLC 的色谱柱填料

• 批准号: 3438532
• 负责人: NEIL D DANIELSON
• 金额: $ 6.05万
• 依托单位: MIAMI UNIVERSITY OXFORD
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3438532
• 关键词: high performance liquid chromatography; polystyrenes; polyvinyls; pyrrolidones

Reaction conditions have been developed to coacervate 600A silica sol by urea-formaldehyde polymerization into 2 mu/m silica microspheres with a pore size of about 300A. After sintering, these particles could be packed into short 3 cm columns for use in high performance liquid chromatography (HPLC). Rapid reversed phase separations of selected mixtures of proteins and peptides using flowrates from 3-4 ml/min were performed in 2 minutes or less. Three areas of study are proposed building on these preliminary data. To permit synthesis of 1 mu/m porous microspheres, use of the Simplex optimization scheme is proposed to understand the interrelationship of reaction variables. Secondly, graft polymerization reactions with vinyl-silica and various monomers such as styrene, acrylonitrile, and vinylpyrrolidone will allow more stable packings to be developed and good chromatographic efficiency to be maintained. Thirdly, selected applications of even shorter 1 cm columns packed with these 1 mu/m particles for clinical chemistry are exemplified.

已经开发了凝聚600 A的反应条件 脲醛聚合成2 μ m/m二氧化硅硅溶胶 微球，孔径约300 A。 烧结后， 这些颗粒可以填充到短的3cm柱中 高效液相色谱法（HPLC）。 快速反转 蛋白质和肽的选定混合物的相分离 使用3-4 ml/min的流速在2分钟内进行，或 少 在此基础上提出了三个研究领域 初步数据。 为了能够合成1 μ/m的多孔 微球，提出了使用单纯形优化方案 了解反应变量的相互关系。 第二，与乙烯基二氧化硅的接枝聚合反应， 各种单体如苯乙烯、丙烯腈和 乙烯基吡咯烷酮将允许开发更稳定的填料 并且保持良好的色谱效率。 第三， 更短的1cm柱的选择应用， 举例说明这些用于临床化学的1 μ m/m颗粒。