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COLUMN PACKINGS FOR FAST HPLC OF BIOMOLECULES

COLUMN PACKINGS FOR FAST HPLC OF BIOMOLECULES
用于生物分子快速 HPLC 的色谱柱填料
批准号:
3438532
负责人:
NEIL D DANIELSON
金额:
$6.05万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1987
资助国家:
美国
项目状态:
已结题
起止时间:
1987-07-01 至 1989-06-30

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中文摘要
翻译
开发了浓缩600A的反应条件
英文摘要
Reaction conditions have been developed to coacervate 600A silica sol by urea-formaldehyde polymerization into 2 mu/m silica microspheres with a pore size of about 300A. After sintering, these particles could be packed into short 3 cm columns for use in high performance liquid chromatography (HPLC). Rapid reversed phase separations of selected mixtures of proteins and peptides using flowrates from 3-4 ml/min were performed in 2 minutes or less. Three areas of study are proposed building on these preliminary data. To permit synthesis of 1 mu/m porous microspheres, use of the Simplex optimization scheme is proposed to understand the interrelationship of reaction variables. Secondly, graft polymerization reactions with vinyl-silica and various monomers such as styrene, acrylonitrile, and vinylpyrrolidone will allow more stable packings to be developed and good chromatographic efficiency to be maintained. Thirdly, selected applications of even shorter 1 cm columns packed with these 1 mu/m particles for clinical chemistry are exemplified.
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Alkylammonium Formate Mobile Phase Modifiers for Protein Liquid Chromatography
  • 批准号:
    7933114
  • 项目类别:
  • 资助金额:
    $3.0万
  • 财政年份:
    2009
  • 负责人:
    NEIL D DANIELSON
  • 依托单位:
Alkylammonium Formate Mobile Phase Modifiers for Protein Liquid Chromatography
  • 批准号:
    7252276
  • 项目类别:
  • 资助金额:
    $21.3万
  • 财政年份:
    2007
  • 负责人:
    NEIL D DANIELSON
  • 依托单位:
ENZYMES STABILIZED BY POLYMERS FOR FIA & HPLC DETECTION
  • 批准号:
    2189354
  • 项目类别:
  • 资助金额:
    $9.79万
  • 财政年份:
    1994
  • 负责人:
    NEIL D DANIELSON
  • 依托单位:
DETECTION OF BIOMOLECULES BY CHEMILUMINESCENCE
  • 批准号:
    3439006
  • 项目类别:
  • 资助金额:
    $10.03万
  • 财政年份:
    1990
  • 负责人:
    NEIL D DANIELSON
  • 依托单位:
海外基金