IGG AFFINITY FOR BACTERIAL ANTIGENS AND PHAGOCYTIC CELLS
IGG AFFINITY FOR BACTERIAL ANTIGENS AND PHAGOCYTIC CELLS
批准号:
3445602
负责人:
Seth V. Hetherington
金额:
$6.24万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1986
资助国家:
美国
项目状态:
已结题
起止时间:
1986-04-01 至 1989-03-31
关键词:
Streptococcus infection Streptococcus pneumoniae Streptococcus pneumoniae vaccine antibacterial antibody antibody specificity bacterial antigens bacterial polysaccharides bactericidal immunity granule human subject immune complex immunoglobulin G immunoglobulin structure immunological substance luminescence microorganism immunology opsonin passive immunization phagocytosis
中文摘要
这项研究计划将开发方法来量化
肺炎链球菌多糖类抗原免疫球蛋白G。
可透析的低聚糖将被制备和提纯以达到平衡
透析。这项技术测量标记的可透析物的结合
用透析膜分离免疫球蛋白G的抗原。Scatchard分析是
用来计算反应的亲和力K,(Ag)(Ab)产额
(阿加布)。根据朗缪尔方程绘制数据会产生一个
总抗体含量的测量。
将开发出测量免疫球蛋白激活吞噬细胞的方法,
包括化学发光、颗粒蛋白释放以及免疫球蛋白结合
到Fc受体。在这些实验中使用的免疫球蛋白将会很好
热凝聚法制备表征的免疫球蛋白低聚物
二硫代二(琥珀酸亚胺基丙酸酯)聚合
凝胶过滤提纯。
评估免疫球蛋白功能的方法将应用于一组
静脉用免疫球蛋白(IVIg)评价疗效
制备方法对免疫球蛋白功能的影响。在静脉注射时
免疫球蛋白已被提倡用于许多临床情况,
它们作为天然免疫球蛋白发挥全部功能的能力还没有
令人信服地展示了。此外,使用一种方法获得的临床结果
准备工作不能外推到另一个,因为这些IVIgG
采用多种酶促和化学改性方法制备。
该项目旨在建立不同的体外标准
静脉注射免疫球蛋白的制备可以与潜在的相关性进行比较。
具有临床疗效。
这些方法也将被用来检查对
多糖类抗原和检验抗肺炎球菌免疫球蛋白的假说
用多价肺炎球菌多糖免疫的受试者中
关于亲和力的同质种群。相关性将是
介于抗原亲和力和光学能力之间。
英文摘要
This research proposal will develop methods to quantitate the affinity of
immunoglobulin G for polysaccharide antigens of Streptococcus pneumoniae.
Dialyzable oligosaccharides will be prepared and purified for equilibrium
dialysis. This technique measures the binding of a labelled dialyzable
antigen to IgG separated by a dialysis membrane. Scatchard analysis is
used to calculate the affinity, K, of the reaction, (Ag) + (Ab) yields
(AgAb). Plotting data according to the Langmuir equation yields a
measurement of total antibody content.
Methods will be developed to measure phagocytic cell activation by IgG,
including chemiluminescence, granule protein release, and also IgG binding
to Fc receptors. The IgG used in these experiments will be well
characterized IgG oligomers prepared by heat aggregation or by
polymerization by dithiobis (succinimidylpropionate) with subsequent
purification by gel filtration.
The methods for assessing IgG function will be applied to a group of
immunoglobulins prepared for intravenous use (IVIgG) to assess the effect
of the method of preparation on IgG function. While intravenous
immunoglobulins have been advocated for a number of clinical situations,
their ability to function to the full extent as native IgG has not been
convincingly shown. In addition, clinical results obtained with one
preparation may not be extrapolated to another since these IVIgGs are
prepared from a variety of enzymatic and chemically modifying methods.
This project aims to establish in vitro standards by which different
preparations of IVIgG may be compared, with the potential for correlation
with clinical efficacy.
These methods will also be used to examine the immune response to
polysaccharide antigens and test the hypothesis that anti-pneumococcal IgG
of subjects immunized with polyvalent pneumococcal polysaccharide is a
homogeneous population with respect to affinity. Correlations will be
drawn between antigen affinity and opsonic capacity.
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IGG AFFINITY FOR BACTERIAL ANTIGENS AND PHAGOCYTIC CELLS
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批准号:3445601
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项目类别:
-
资助金额:$5.14万
-
财政年份:1986
-
负责人:Seth V. Hetherington
-
依托单位:
IGG AFFINITY FOR BACTERIAL ANTIGENS AND PHAGOCYTIC CELLS
-
批准号:3445603
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项目类别:
-
资助金额:$6.06万
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财政年份:1986
-
负责人:Seth V. Hetherington
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依托单位:
海外基金