SPECIFIC ANTIGENS OF ATYPICAL MYCOBACTERIA

这项提议的核心是新发现的、非凡的 脂寡糖抗原，在非典型(非结核)中普遍存在 分枝杆菌，可能存在于结核分枝杆菌和 麻风分枝杆菌来自堪萨斯分枝杆菌的菌株具有 由含有海藻糖的糖苷“核心”调节，由物种特异性调节 糖和酰基取代基。最先进的化学分析(例如360 MHZ~1H-和~(13)C-核磁共振；气-液-质联用；快原子 轰击/质谱学)将被应用，探测各种 含海藻糖的“核心”与种属间的关系 糖和酰基对“核”起作用。丙酮酸的分析 猪体内含酸性“C-霉苷”糖肽脂(GPL)抗原 禽分枝杆菌/胞内分枝杆菌/鞭毛分枝杆菌 复合体将继续与对它们的生物合成的探测相一致， 基于对它们起源于水溶性糖肽的观察 对D环丝氨酸敏感。溶原性转化在细胞周期中的作用 自然界中含有糖肽脂的多种血清型将 被探索。此外，临床应用中还存在种属特异性 糖脂，加入到酶联免疫吸附分析中，在 病原体鉴定和疾病的直接血清诊断 继续。生理学功能及其在发病/持续性中的作用 物种特有的脂寡糖/糖肽脂类将是 重点探讨了惰性类脂胶囊的理论。

The centerpiece of this proposal is the new found, extraordinary lipooligosaccharide antigens, ubiquitous among atypical (nontuberculous) mycobacteria and perhaps present in Mycobacterium tuberculosis and Mycobacterium leprae. Those from Mycobacterium kansasii are characterized by a glycosidic trehalose-containing "core", modulated by species-specific sugars and acyl substituents. State-of-the-art chemical analysis (e.g. 360 MHz 1H- and 13C-NMR; gas liquid chromatography/mass spectrometry; fast atom bombardment/mass spectrometry) will be applied, probing the variety of trehalose-containing "cores" and the relationship of species-specific sugars and acyl functions to the "cores". Analysis of the pyruvic acid-containing "C-mycoside" glycopeptidolipid (GPL) antigens of the Mycobacterium avium/Mycobacterium intracellulare/Mycobacterium scrofulaceum complex will continue in concert with a probing of their biosynthesis, based on observations that they originate in water-soluble glycopeptides and are susceptible to D cycloserine. The role of lysogenic conversion in the multiplicity of glycopeptidolipid-containing serotypes in nature will be explored. In addition, the clinical application of the species-specific glycolipids, incorporated into enzyme linked immunosorbent assays, in identification of pathogens and direct serodiagnosis of disease will continue. The physiological function and role in pathogenesis/persistence of the species-specific lipooligosaccharides/glycopeptidolipids will be explored with emphasis on the theorm of an inert lipoidal capsule.