DYNAMICS OF CYCLIC NUCLEOTIDE METABOLISM IN SPERM

精子中环核苷酸代谢的动力学

基本信息

  • 批准号:
    3447967
  • 负责人:
  • 金额:
    $ 4.85万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1983
  • 资助国家:
    美国
  • 起止时间:
    1983-12-01 至 1986-11-30
  • 项目状态:
    已结题

项目摘要

A new technique, based on the property of phosphodiesterases to promote P-O bond cleavage during the hydrolysis of cyclic 3 feet, 5 feet nucleotides and the insertion of 18-O from [18-O]water into the phosphoryls of the 5 feet-nucleotide product will be used to monitor the dynamics of cAMP and cGMP metabolism in intact sea urchin sperm. By this new analytical approach information has recently been obtained indicating that in addition to the allosteric mode of cyclic nucleotide action involving protein kinase activation, there may be an influence on certain cell functions that are related to the hydrolysis of cyclic nucleotides. This relationship was uncoverd in the visual retina where it was found that light stimuli over a 4 log range of intensities promote incremental increases in cGMP hydrolytic flux. These light-induced increases in cGMP metabolic flux were not associated with any changes in cellular cGMP steady state levels, indicating that the increased rate of cGMP hydrolysis is tightly coupled to an equal increase in cGMP synthesis. This increase in cGMP metabolic flux has been postulated to be integral to a mechanism for releasing bound calcium. One of the intensions of the experiments described in this proposal is to determine if the modification of certain metabolic and functional events in sperm are also associated with this newly disclosed modification of cyclic nucleotide metabolism that can only be uncovered by the type of metabolic measurements that the 18-O labeling procedure provides. Obtaining information regarding the fundamental dynamics of cGMP and cAMP metabolism in sperm represents the primary goal of the studies to be conducted. Several biological processes in sea urchin sperm including the acrosome reaction, respiration, motility and ion transport are activated or elicited by specific factors derived from sea urchin eggs. The actions of these egg factors have been found to be associated with alterations in cyclic nucleotide metabolism but the precise involvement of cyclic nucleotides in influencing sperm events is unclear. The information that will be obtained from the 18-O labeling studies proposed should help to provide some new insights into the role that cyclic nucleotides and alterations in their metabolism may play in modulating and/or promoting certain of the cellular events in sperm. The measurement of 18-O labeling of adenine and guanine nucleotide Alpha, Beta, and Lambda-phosphoryls within the intact cells also provides the potential for examining overall nucleotide and energy metabolism in relation to the processes that can be promoted in these cells.
一种新的技术,基于磷酸二酯酶促进P-O 环状3英尺、5英尺核苷酸水解过程中的键断裂 和插入18-O从[18-O]水到磷酰基的5 足核苷酸产物将用于监测cAMP的动力学, 完整海胆精子的cGMP代谢。 通过这一新的分析 最近获得的方法信息表明,此外, 涉及蛋白激酶的环核苷酸作用的变构模式 激活,可能会对某些细胞功能产生影响, 与环核苷酸的水解有关。 这种关系 在视觉视网膜中发现, 强度的4个对数范围促进cGMP水解的增量增加 通量 这些光诱导的cGMP代谢通量的增加并不 与细胞cGMP稳态水平的任何变化相关, 表明cGMP水解速率的增加与 cGMP合成的同等增加。 cGMP代谢通量的增加 被认为是释放束缚的机制的组成部分, 钙 这篇文章中描述的实验的一个目的是, 建议是确定是否修改某些代谢和 精子中的功能事件也与这种新披露的 环核苷酸代谢的修饰,只能通过 18-O标记程序 提供. 获取cGMP基本动力学信息 精子中的cAMP代谢代表了研究的主要目标, 进行。 海胆精子包括顶体在内的几个生物学过程 反应、呼吸、运动和离子转运被激活或引发 源自海胆卵的特殊因子。 这些鸡蛋的行为 已发现的因素与周期性变化有关, 核苷酸代谢,但环核苷酸的精确参与, 影响精子活动的因素尚不清楚。 将获得的信息 从18-O标记的研究建议应该有助于提供一些新的 深入了解环核苷酸的作用和它们的改变, 代谢可以在调节和/或促进某些细胞代谢中起作用。 精子中的事件 腺嘌呤和鸟嘌呤的18-O标记测定 完整细胞内的核苷酸α、β和λ-磷酰基也 提供了检查总体核苷酸和能量的可能性 代谢的过程,可以促进这些细胞。

项目成果

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TIMOTHY Francis WALSETH其他文献

TIMOTHY Francis WALSETH的其他文献

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{{ truncateString('TIMOTHY Francis WALSETH', 18)}}的其他基金

Regulation of the Cyclic ADP-Ribose Signaling System
环状 ADP-核糖信号系统的调节
  • 批准号:
    7513855
  • 财政年份:
    2007
  • 资助金额:
    $ 4.85万
  • 项目类别:
REGULATION OF CYCLIC ADP RIBOSE SIGNALING SYSTEM
环状 ADP 核糖信号系统的调控
  • 批准号:
    6338716
  • 财政年份:
    2000
  • 资助金额:
    $ 4.85万
  • 项目类别:
NAADP SIGNALING IN MAMMALIAN SYSTEMS
哺乳动物系统中的 NAADP 信号传导
  • 批准号:
    6151224
  • 财政年份:
    1999
  • 资助金额:
    $ 4.85万
  • 项目类别:
REGULATION OF CYCLIC ADP RIBOSE SIGNALING SYSTEM
环状 ADP 核糖信号系统的调控
  • 批准号:
    6201645
  • 财政年份:
    1999
  • 资助金额:
    $ 4.85万
  • 项目类别:
NAADP SIGNALING IN MAMMALIAN SYSTEMS
哺乳动物系统中的 NAADP 信号传导
  • 批准号:
    2727917
  • 财政年份:
    1999
  • 资助金额:
    $ 4.85万
  • 项目类别:
REGULATION OF CYCLIC ADP RIBOSE SIGNALING SYSTEM
环状 ADP 核糖信号系统的调控
  • 批准号:
    6104194
  • 财政年份:
    1998
  • 资助金额:
    $ 4.85万
  • 项目类别:
ROLE OF CYCLIC ADP-RIBOSE IN CALCIUM DISPOSITION IN NG108-15 CELLS
环状 ADP-核糖在 NG108-15 细胞钙沉积中的作用
  • 批准号:
    6237945
  • 财政年份:
    1997
  • 资助金额:
    $ 4.85万
  • 项目类别:
DYNAMICS OF CYCLIC NUCLEOTIDE METABOLISM IN SPERM
精子中环核苷酸代谢的动力学
  • 批准号:
    3447966
  • 财政年份:
    1983
  • 资助金额:
    $ 4.85万
  • 项目类别:
ROLE OF CYCLIC ADP-RIBOSE IN CALCIUM DISPOSITION IN NG108-15 CELLS
环状 ADP-核糖在 NG108-15 细胞钙沉积中的作用
  • 批准号:
    5209692
  • 财政年份:
  • 资助金额:
    $ 4.85万
  • 项目类别:

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Microglia adenine nucleotides and hypoxia
小胶质细胞腺嘌呤核苷酸和缺氧
  • 批准号:
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  • 财政年份:
    2005
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  • 项目类别:
Microglia adenine nucleotides and hypoxia
小胶质细胞腺嘌呤核苷酸和缺氧
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Microglia adenine nucleotides and hypoxia
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  • 批准号:
    7433732
  • 财政年份:
    2005
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Microglia adenine nucleotides and hypoxia
小胶质细胞腺嘌呤核苷酸和缺氧
  • 批准号:
    6967573
  • 财政年份:
    2005
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    $ 4.85万
  • 项目类别:
Microglia adenine nucleotides and hypoxia
小胶质细胞腺嘌呤核苷酸和缺氧
  • 批准号:
    7615702
  • 财政年份:
    2005
  • 资助金额:
    $ 4.85万
  • 项目类别:
METAL MEDIATED MOLECULAR AGGREGATES OF GUANINE AND ADENINE NUCLEOTIDES
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    6655269
  • 财政年份:
    2002
  • 资助金额:
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METAL MEDIATED MOLECULAR AGGREGATES OF GUANINE AND ADENINE NUCLEOTIDES
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  • 批准号:
    6495409
  • 财政年份:
    2001
  • 资助金额:
    $ 4.85万
  • 项目类别:
METAL MEDIATED MOLECULAR AGGREGATES OF GUANINE AND ADENINE NUCLEOTIDES
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  • 批准号:
    6492840
  • 财政年份:
    2001
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    $ 4.85万
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METAL MEDIATED MOLECULAR AGGREGATES OF GUANINE AND ADENINE NUCLEOTIDES
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  • 批准号:
    6346184
  • 财政年份:
    2000
  • 资助金额:
    $ 4.85万
  • 项目类别:
METAL MEDIATED MOLECULAR AGGREGATES OF GUANINE AND ADENINE NUCLEOTIDES
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  • 批准号:
    6354089
  • 财政年份:
    2000
  • 资助金额:
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