DRUG-INDUCED NEPHROTOXICITY: A CELL CULTURE APPROACH

药物引起的肾毒性：细胞培养方法

• 批准号: 3447342
• 负责人: MARY ANN SENS
• 金额: $ 4.75万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-23 至 1989-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3447342
• 关键词: aminoglycoside antibiotics; cell bank /registry; cell population study; cytotoxicity; dosage; drug adverse effect; growth media; histopathology; human tissue; image processing; ion transport; kidney function; kidney pharmacology; lysozyme; radiotracer; renal toxin; renal tubule; streptomycin

The long-term objective of the proposed research plan is the formation of an in vitro cell culture model system for the study of aminoglycoside-induced nephrotoxicity. The foundation for this model system rests on a newly established cell culture of the human renal proximal convoluted tubule. Preliminary data has clearly shown that these cells respond to aminoglycoside exposure by formation of lysosomal myeloid bodies. The specific aims of the proposed research is initially to reinforce preliminary findings suggesting that aminoglycoside toxicity and myeloid body formation do not correlate directly. Rather, it appears that the least toxic aminoglycosides are the most efficient at inducing myeloid body formation. Additional specific aims center on an overall assessment of lysosomal function and ion transport alterations elicited by exposure to the aminoglycosides. The above parameters will be further perturbed to enhance and/or correct these alterations by changing the cation composition of the serum-free growth medium in which these cells grow. An understanding of the mechanism of aminoglycoside-induced nephrotoxicity may lead to clinical improvements in hospitalized patients suffering from sepsis.

提出的研究计划的长期目标是形成

项目成果

Growth characteristics of cultured human proximal tubule cells exposed to aminoglycoside antibiotics.

暴露于氨基糖苷类抗生素的培养的人近曲小管细胞的生长特征。

• 发表时间: 1989
• 期刊: Annals of clinical and laboratory science
• 影响因子: 0.8
• 作者: Sens,MA;Hazen-Martin,DJ;Blackburn,JG;Hennigar,GR;Sens,DA
• 通讯作者: Sens,DA