EFFECTS OF MITOGENS ON MUSCLE GROWTH & GENE EXPRESSION

有丝分裂原对肌肉生长的影响

• 批准号: 3456870
• 负责人: NEVIS L FREGIEN
• 金额: $ 11.16万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3456870
• 关键词: cell cell interaction; cell differentiation; cell growth regulation; complementary DNA; endonuclease; epidermal growth factor; fibroblast growth factor; gene expression; gene induction /repression; genetic library; genetic manipulation; genetic regulation; histogenesis; laboratory mouse; mitogens; myoepithelial cell; myogenesis; nucleic acid hybridization; radiotracer; regeneration; tissue /cell culture

The principal objective of this project is to understand, at the molecular level, two main aspects of muscle growth and development. The first set of experiments will use gene transfer technology to study the control of myoblast growth and differentiation by growth factors. The genes encoding the epidermal growth factor receptor and the basic fibroblast growth factor will be inserted and expressed in myogenic cell lines. These cells will then be tested to see if these genetic alterations cause the expected or unexpected changes in the normal program of myoblast proliferation and differentiation. Further experiments, including a molecular genetic approach to the isolation of the fibroblast growth factor receptor, will be done to elucidate the mechanism of growth factor activity in a differentiation cell system. A second set of experiments will be done using, subtraction cDNA cloning technology, to identify and isolate myoblast genes which are regulated during the determination and proliferation stages of muscle development. Genes which are expressed in response to fibroblast growth factor stimulated proliferation will be cloned. These genes will be characterized to identify proteins which are required during the proliferative stage of myogenesis and to further understand the mechanism of growth factor response. The genes which are activated during the commitment of embryonic cells to the myoblast lineage will also be isolated. These genes will provide the initial characterization of the changes in genetic activity during the period of cell fate determination and the factors controlling this poorly understood stage of development.

这个项目的主要目标是了解

项目成果

DNA-mediated transformation of N-acetylglucosaminyltransferase I activity into an enzyme deficient cell line.

DNA 介导的 N-乙酰氨基葡萄糖转移酶 I 活性转化为酶缺陷细胞系。

• DOI: 10.1016/0006-291x(89)90029-6
• 发表时间: 1989
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Ripka,J;Pierce,M;Fregien,N
• 通讯作者: Fregien,N

Co-transformation of Lec 1 CHO cells with N-acetylglucosaminyltransferase 1 activity and a selectable marker.

Lec 1 CHO 细胞与 N-乙酰氨基葡萄糖转移酶 1 活性和选择标记的共转化。

• DOI: 10.1002/jcb.240420302
• 发表时间: 1990
• 期刊: Journal of cellular biochemistry
• 影响因子: 4
• 作者: Ripka,J;Pierce,M;Fregien,N
• 通讯作者: Fregien,N