DIFFERENTIATION OF TERATOCARCINOMA CELLS: REGULATION

畸胎癌细胞的分化：调节

• 批准号: 3458209
• 负责人: SHO-YA Y WANG
• 金额: $ 9.22万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT ALBANY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-01 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3458209
• 关键词: DNA; cell differentiation; collagen; cytogenetics; early embryonic stage; gene expression; genetic mapping; genetic transcription; globin; hematopoietic stem cells; laminin; molecular cloning; neoplasm /cancer genetics; neoplastic cell; nucleic acid probes; nucleic acid sequence; radiotracer; retinoate; simian virus 40; teratoma; tissue /cell culture; transfection

The goals of this project are (1) to understand the structure of two genes of which expression is controlled by retinoic acid and (2) to identify cis-acting DNA elements involved in the collagen IV and another differentiation gene during F9 stem cell differentiation. I plan to examine the restriction maps of these two F9 differentiation specific genes, identify the structural regions and more specifically the '5 end transcription cap sites. Different sizes of '5 flanking regions of two genes will be linked to Beta-globin structure gene of which promotor and '3 end of gene has been deleted. Transfection assays by using hybrid gene-SV40 vector as donor will be performed in F9 stem cells. The retinoic acid sensitive DNA elements will be determined in the transfected cells when higher degree of expression of Beta-globin gene is detected at the differentiated stage of F9 cells. These series of experiments will allow me to identify the retinoic acid regulated sequences directly or indirectly. Retinoic acid has been implicated in the control of differentiation in epithelial tissues and in some other cell types. The mechanism by which retinoic acid induce differentiation of teratocarcinoma cells is not well understood. Investigation in this field speculate that retinoic acid may act in a manner analogous to steroid hormones. However, the experimental data is too preliminary to make any firm conclusions. The studies of differentiation genes by using molecular cDNA as probe and further identifications of retinoic acid regulatory elements will inform us of the underlying mechanism of the action of retinoic acid. In addition, the understanding of early embryonic differentiation may also facilitate the understanding of oncogenesis which results from dedifferentiation in some cases.

这个项目的目标是：(1)了解两个基因的结构

项目成果

Structure of the gene and its retinoic acid-regulatory region for murine J6 serpin. An F9 teratocarcinoma cell retinoic acid-inducible protein.

鼠 J6 丝氨酸蛋白酶抑制剂的基因结构及其视黄酸调节区。

• 发表时间: 1992
• 期刊: The Journal of biological chemistry
• 作者: Wang,SY

A retinoic acid-inducible GATA-binding protein binds to the regulatory region of J6 serpin gene.

视黄酸诱导型 GATA 结合蛋白与 J6 丝氨酸蛋白酶抑制剂基因的调控区结合。

• 发表时间: 1994
• 期刊: The Journal of biological chemistry
• 作者: Wang,SY