FACTORS REGULATING PROPERTIES OF SKELETAL MUSCLE

骨骼肌特性的调节因素

• 批准号: 3457127
• 负责人: PETER J REISER
• 金额: $ 0.11万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1992-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3457127
• 关键词: cardiovascular stress test; chick embryo; chickens; exercise; fibrogenesis; gel electrophoresis; hormone binding protein; hormone regulation /control mechanism; isozymes; laboratory rabbit; laboratory rat; membrane proteins; muscle cells; muscle contraction; muscle pharmacology; muscle proteins; muscle stimulant; muscle tension; myofibrils; myosins; nervous system prosthesis; neural facilitation; protein structure function; reflex; stretch reflex; striated muscles; western blottings

Contractile and biochemical properties of skeletal muscles of immature and adult animals are dependent on the activity patterns of the muscles in accordance with normal functional demands. The relationships between specific protein compositions and the contractile properties of individual muscle cells are beginning to be understood. However, the physiological factors that regulate myofibrillar protein composition and, therefore, contractile properties during development and in adult muscle are less well understood. The long-term goal of this project is to determine which factors are important in the regulation of skeletal muscle activity and the effects that these factors have on the expression of contractile and biochemical characteristics of single muscle cells. The activity of skeletal muscle will be experimentally altered using functional overload, hindlimb suspension to induce hypokinesia and chronic in situ stimulation using different patterns of electrical stimulation. Contractile and biochemical measurements will be made on transformed muscles and compared to those obtained from control muscles. The contractile properties to be studied are the maximal velocity of shortening, the tension generating ability and tension/pea relationships of single, skinned muscle fibers. The protein composition will be assessed using ultrasensitive gel electrophoresis and immunoblotting techniques with monoclonal antibodies against specific myosin heavy chains. Measurements will be performed on single muscle fibers that have had their surface membranes removed using a chemical skinning procedure to allow direct control of activation with Ca2+. In those cases where the individual fibers are too small for satisfactory measurements (e.g., embryonic muscle), whole muscles will be studied. The design of the experiments will permit the determination of molecular structure-function relationships in muscle by identifying associations between specific contractile properties and protein compositions of developing, adult and transformed muscle An examination of the adaptability of skeletal muscle In response to alterations in functional demands (as during chronic exercise) should improve our understanding of the physiological regulation of skeletal muscle performance. This information should be extremely valuable for the design of neural prostheses which are used to facilitate or restore motor function in certain clinical situations.

未成熟和未成熟骨骼肌的收缩和生化特性 成年动物依赖于肌肉的活动模式 符合正常的功能需求。之间的关系 特定的蛋白质组成和个体的收缩特性 人们开始了解肌肉细胞。然而，生理 调节肌原纤维蛋白组成的因素，因此， 发育期间和成年肌肉的收缩特性较差 明白了。该项目的长期目标是确定哪些 因素对骨骼肌活动的调节很重要 这些因素对收缩和表达的影响 单个肌细胞的生化特征。的活动 骨骼肌将通过功能超负荷进行实验性改变， 后肢悬吊诱发运动功能减退和慢性原位刺激 使用不同模式的电刺激。收缩和 将对转化后的肌肉进行生化测量并进行比较 那些从控制肌肉获得的。的收缩特性为 研究了缩短的最大速度、产生的张力 单个带皮肌纤维的能力和张力/豌豆关系。这 将使用超灵敏凝胶评估蛋白质成分 单克隆抗体电泳和免疫印迹技术 对抗特定的肌球蛋白重链。测量将在 使用去除其表面膜的单肌纤维 化学剥皮程序可直接控制活化 钙2+。在单根纤维太小而无法使用的情况下 令人满意的测量（例如胚胎肌肉），整个肌肉将 研究过。实验的设计将允许确定 通过识别肌肉中的分子结构-功能关系 特定收缩特性与蛋白质之间的关联 发育中、成年和转化肌肉的成分 骨骼肌对变化的适应性 功能需求（如长期锻炼期间）应该改善我们的 了解骨骼肌的生理调节 表现。这些信息对于设计来说应该非常有价值 用于促进或恢复运动功能的神经假体 在某些临床情况下。