AN ONCOGENE SENSITIVE REGULATION OF DIFFERENTIATION

癌基因敏感的分化调节

• 批准号: 3458122
• 负责人: PAUL V CHERINGTON
• 金额: $ 10.95万
• 依托单位: TUFTS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 1994-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3458122
• 关键词: adipocytes; athymic mouse; autoradiography; binding proteins; cell differentiation; cell growth regulation; computer assisted sequence analysis; gel electrophoresis; gene expression; gene mutation; genetic manipulation; genetic regulation; growth factor; immunofluorescence technique; immunoprecipitation; molecular cloning; molecular genetics; morphology; nucleic acid probes; oncogenes; protein biosynthesis; protein structure function; radionuclides; tissue /cell culture

Oncogenes have, in many cases, been shown to interfere with as well as growth regulation. Very little is known, however, about differentiation the relationship between these oncogene functions. The goal of the proposed project is to determine the mechanism of SV40 large T antigen in a well defined, novel assay for differentiation. In order to determine, at a molecular level, how T antigen blocks differentiation we will perform the following experiments: 1. A detailed structure-function analysis of SV40 T antigen block of adipocyte differentiation using existing mutants. 2. Determine whether Sv40 T antigen blocks adipocyte by differentiation interfering with growth factor regulated cell cycle arrest. 3. Determine how SV40 T antigen suppresses the expression of an adipocyte-specific gene family.

在许多情况下，癌基因也被证明会干扰 作为生长调节。 然而，人们对 分化这些癌基因功能之间的关系。 该项目的目标是确定 SV40大T抗原在一种定义明确的新型检测中用于 分化 为了在分子水平上确定 T抗原阻断分化，我们将执行以下操作 实验： 1. SV40 T抗原阻断剂的结构与功能分析 脂肪细胞分化的研究。 2. 通过以下方法确定Sv40 T抗原是否阻断脂肪细胞： 分化干扰生长因子调节的细胞周期 逮捕了 3. 确定SV40 T抗原如何抑制 脂肪细胞特异性基因家族。