INPUT/OUTPUT RELATIONSHIPS IN VISUAL CORTEX

视觉皮层的输入/输出关系

• 批准号: 3465670
• 负责人: Gillian Einstein
• 金额: $ 11.2万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-01 至 1993-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3465670
• 关键词: afferent nerve; axon; cats; dendrites; electron microscopy; immunocytochemistry; neural information processing; neuroanatomy; nontherapeutic iontophoresis; pons; superior colliculus; visceral afferent nerve; visual cortex; visual feedback; visual pathways

The proposed experiments investigate the relationship between the pattern of termination of various types of afferents and the pattern of dendritic arborization of neurons that project to different targets. The two specific questions being posed are: 1) Do cortical neurons with different efferent targets have different patterns of dendritic arborization? 2) How do different sources of afferents terminate with respect to those patterns of arborization? This study will be carried-out in cat Posterior Medial Lateral Suprasylvian Sulcus (PMLS), a visual area with diverse sources of afferents as well as multiple cortical and subcortical efferent targets. The first question will be approached using a lightly fixed slice preparation to intracellularly inject layer V neurons that have been retrogradely labeled from three regions known to play different roles in the processing of visual information, the superior colliculus, pons, and ipsilateral striate cortex. The dendritic morphologies of neurons that project to these areas will be compared in order to determine if there are differences in patterns of afferent sampling. Neurons that project to two of these sites, via collaterals, will also be retrogradely labeled and intracellularly injected in order to determine if they are morphologically distinct from the neurons that project to only one of the two sites. The second question will be approached by making separate sets of iontophoretic injections of anterograde tracer into three regions thought to be responsible for the unique physiological properties of PMLS neurons, the lateral posterior/pulvinar complex, the intralaminar nuclei, and the ipsilateral striate cortex. Anterogradely filled axons and their boutons will be drawn and their distribution compared with the different dendritic patterns of layer V projecting neurons. By studying the relationship of input to output neurons in PMLS, it is hoped that the results will contribute to an understanding this region's role in visuomotor behavior.

所提出的实验研究了之间的关系 各种类型传入的终止模式和 投射到的神经元的树突状树枝化模式 不同的目标。 提出的两个具体问题是：1） 具有不同传出目标的皮层神经元是否有不同的 树突状树枝化的模式？ 2）不同来源如何 的传入终止于这些模式 树枝化？ 这项研究将在猫后部进行 内侧外侧上裂 (PMLS)，一个视觉区域 不同的传入源以及多个皮质和 皮层下传出目标。 第一个问题是 使用轻微固定的切片制剂来接近 细胞内注射已逆行的V层神经元 标记为已知在生物多样性中发挥不同作用的三个区域 视觉信息处理、上丘、脑桥、 和同侧纹状皮层。 的树突形态 投射到这些区域的神经元将被比较，以便 确定传入模式是否存在差异 采样。 投射到其中两个位点的神经元，通过 抵押品，也将被逆行标记和细胞内 注射以确定它们是否在形态上不同 来自仅投射到两个位点之一的神经元。 这 第二个问题将通过制作单独的组来解决 离子电渗疗法将顺行示踪剂注射到三个区域 被认为是造成独特生理特性的原因 PMLS 神经元、外侧后/枕复合体、 层内核和同侧纹状皮层。 顺行填充的轴突及其纽扣将被绘制并 它们的分布与不同的树突模式相比 V 层投射神经元。 通过研究关系 输入到 PMLS 中的输出神经元，希望结果 有助于理解该区域在视觉运动中的作用 行为。