PHYSICOCHEMICAL & BIOANALYTICAL STUDIES USING MLC

这项工作的目的是探索高度有前途的 胶束液相色谱作为一种强大的 化学分析和物理化学研究技术 生物医学研究 该提案涉及一个系统的 胶束液相色谱的应用研究： 生物重要分子的理化性质， 环境污染物;研究的能量学和 溶质-胶束相互作用的机理及其对 胶束液相色谱的保留和选择性， 快速灵敏的生化分析方法 化合物. 这三个项目共提出六个项目 主要领域是目前非常感兴趣的，因为他们 由于其内在的重要性，并由于其广泛的影响， 分离科学以外的学科范围;从 从药物化学到生物技术。 在第一部分中，目的是将胶束LC应用于 有机化合物疏水特性的定量。 疏水性的表征具有诊断和 在药物设计等不同学科中的预测价值， 毒理学和环境监测 胶束LC提供清晰的 与传统方法和RPLC相比， 用于估算疏水性的二元溶剂。 在本研究的第二部分，目的是更好地定义 胶束液相色谱保留和选择性的物理化学基础 通过精心设计的实验， 溶质-胶束相互作用的意义。 最后，在第三部分，旨在论证《红楼梦》的独特性 胶束LC用于化学分析的能力， 特别是在解决生物分析问题方面的优势。

The purpose of this work is to explore the highly promising capabilities of Micellar Liquid Chromatography as a powerful technique for chemical analysis and physico-chemical studies in biomedical research. The proposal involves a systematic investigation of the use of Micellar LC for: characterization of physio-chemical properties of biologically important molecules and environmental pollutants; the study of the energetics and mechanisms of solute-micelle interactions and their effects on retention and selectivity in Micellar LC, and the development of rapid and sensitive methods for the analysis of biochemical compounds. A total of six projects are proposed in these three main areas which are of much current interest due to their intrinsic significance, and because of their impact on a broad range of disciplines other than separation sciences; from pharmaceutical chemistry to biotechnology. In the first part, the aim is to apply micellar LC for the quantitation of hydrophobic character of organic compounds. Characterization of hydrophobicity has both a diagnostic and predictive value in various disciplines such as drug design, toxicology, and environmental monitoring. Micellar LC offers clear advantages over both classical methods and RPLC with traditional binary solvents for estimation of hydrophobicity. In the second part of this study, the aim is to better define the physico-chemical basis of retention and selectivity in micellar LC through a careful design of experiments to highlight the significance of solute-micelle interactions. Finally, in the third part, the aim is to demonstrate the unique capabilities of micellar LC for chemical analysis which are especially advantages in solving bioanalytical problems.