MATRIX ISOLATION INFRARED SPECTROSCOPY OF MASS IONS

质量离子的矩阵隔离红外光谱

• 批准号: 3467105
• 负责人: ROBERT L WHITE
• 金额: $ 7.94万
• 依托单位: UNIVERSITY OF OKLAHOMA
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3467105
• 关键词: chemical structure function; cryoscience; infrared spectrometry; interferometry; mass spectrometry

Mass spectrometry has evolved into an important analysis technique for biologically important species. The development of soft ionization methods has permitted mass analysis for large molecules and tandem mass spectrometry has been employed to obtain structural information for these molecules. Unfortunately, mass spectrometry alone cannot provide unequivocal structure elucidation. However, infrared spectroscopy provides complementary information which could be correlated with mass spectrometry measurements to permit unequivocal structure determination. The goal of this proposal is to develop instrumentation and methodologies for obtaining infrared spectra for mass selected ions formed by a mass spectrometer ion source. The proposed apparatus combines the advantages of mass selection using a quadrupole mass filter with the analyte concentrating feature of matrix isolation. Ions to be studied will be selected using a quadrupole mass filter and then deposited into an argon matrix at cryogenic temperatures. Infrared analysis will be performed by Fourier transform infrared spectroscopy. The apparatus will be tested using well-behaved systems and then applied to structure elucidation for various biologically important parent and daughter ions. Infrared spectral information will be correlated with mass spectrometric measurements in order to facilitate unequivocal structure elucidation.

质谱分析已经发展成为一种重要的分析方法 生物学上重要物种的技术。 的发展 软电离方法允许对大的样品进行质量分析， 分子和串联质谱已经被用于 获取这些分子的结构信息。 不幸的是， 单靠质谱法不能提供明确的结构 澄清。 然而，红外光谱提供了 可能与质量相关的补充信息 光谱测量允许明确的结构 保持战略定力 该提案的目标是发展 获得红外光谱的仪器和方法 用于由质谱仪离子源形成的质量选择离子。 所提出的装置结合了质量 使用具有分析物的四极质量过滤器进行选择 基质隔离的集中特征。 待研究的离子将 使用四极滤质器进行选择，然后沉积到 在低温下的氩基质。 红外分析将 通过傅里叶变换红外光谱法进行。 的 仪器将使用性能良好的系统进行测试，然后 应用于各种生物学上重要的化合物的结构解析 母离子和子离子。 红外光谱信息将被 与质谱测量相关， 有助于明确的结构阐明。