PARACRINE CONTROL OF FOLLICULAR GROWTH AND OVULATION

这项研究的长期目标是了解精细控制 卵巢功能的变化，从而提供控制生育的机会 并缓解女性某些形式的不孕不育。目前的维度是 了解卵泡发育和排卵的旁分泌控制 通过对当地生产和一些生物的研究 前列腺素(PGs)和松弛素在卵泡中的共同作用 猪和老鼠的关系。他们的生产地点和地点的分布 它们在猪和大鼠卵泡中的原位受体将被确定 在整个卵泡发育和排卵抑制之后。这个 调节PGE2、PGF2Alpha和松弛素局部产生的因素 将对分离的颗粒细胞、膜细胞以及卵泡壁进行研究。 在试管中。旁分泌松弛素在猪体内的内源性释放 卵泡液将从RIA测量中推断出来，并与 卵泡发育过程中PGE2和PGF2Alpha的水平。 PGE2、PGF2Alpha和松弛素共同的部分生物学作用， 参与胶原重塑的酶的释放，将与 它们各自的受体在卵泡中的定位 发展。方法论将包括确定 免疫细胞化学和激素受体原位产生激素 定量光学显微镜放射自显影定位。 卵泡液和体外培养上清液中的激素浓度 卵泡组织的培养将通过常规方法进行测量 放射免疫分析。前列腺素和松弛的生物学作用 共同之处将通过测量纤溶酶原的释放来确定 体外分离细胞的激活剂和胶原酶。

The long term objective of this research is to understand the fine controls of ovarian function and thus to provide opportunities to control fertility and alleviate some forms infertility in women. The present dimension is to understand the paracrine control of follicular development and ovulation through the study of the local production and some of the biological actions in common between prostaglandins (PGs) and relaxin in the follicle of the pig and rat. The distribution of their sites of production and their receptors in situ in pig and rat follicles will be determined throughout follicular development and after inhibition of ovulation. The factors modulating this local production of PGE2, PGF2Alpha and relaxin by isolated granulosa and theca cells as well as follicle wall will be studied in vitro. The endogenous release of paracrine relaxin into porcine follicular fluid will be deduced from measurements by RIA and compared with PGE2 and PGF2Alpha levels during follicular development. Selected biological actions in common between PGE2, PGF2Alpha and relaxin, the release of enzymes involved in collagen remodelling, will be related to the localization of their respective receptors in the follicle during development. Methodology will include the determination of the sites of hormone production in situ by immunocytochemistry and hormone receptor localization by quantitative light microscopic autoradiography. Hormone concentrations in follicular fluids and in media from in vitro cultures of follicular tissue will be measured by conventional radioimmunoassay. The biological actions of the prostaglandins and relaxin in common will be determined by measuring the release of plasminogen activator and collagenase by isolated cells in vitro.